Twenty years of qPCR: A mature technology?

Alessandro Raso, Roberto Biassoni

Research output: Contribution to journalArticlepeer-review


Quantitative PCR is the “gold standard” technology to quantify nucleic acids and, since the first report describing real-time PCR detection in 1993, its use has been grown exponentially. More recent technological advancements have extended the field of applications ranging from high-resolution melting detection to digital PCR. Nowadays, it is a very accessible technique, but some pitfalls should be overcome in order to achieve robust and reliable analysis.

Original languageEnglish
JournalMethods in molecular biology (Clifton, N.J.)
Publication statusPublished - 2014


  • dPCR
  • Dye-labeled probe
  • HRM
  • Intercalating dye
  • qPCR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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