TWIST1 plays a pleiotropic role in determining the anaplastic thyroid cancer phenotype

Paolo Salerno, Ginesa Garcia-Rostan, Sara Piccinin, Tammaro Claudio Bencivenga, Gennaro Di Maro, Claudio Doglioni, Fulvio Basolo, Roberta Maestro, Alfredo Fusco, Massimo Santoro, Giuliana Salvatore

Research output: Contribution to journalArticlepeer-review

Abstract

Context: Anaplastic thyroid carcinoma (ATC) is one of the most aggressive human tumors; it is characterized by chemoresistance, local invasion, and distant metastases. ATC is invariably fatal. Objective: The aim was to study the role of TWIST1, a basic helix-loop-helix transcription factor, in ATC. Design: Expression of TWIST1 was studied by immunohistochemistry and real-time PCR in normal thyroids and well-differentiated, poorly differentiated, and ATC. The function of TWIST1 was studied by RNA interference in ATC cells and by ectopic expression in well-differentiated thyroid carcinoma cells. Results: ATCs up-regulate TWIST1 with respect to normal thyroids as well as to poorly and welldifferentiated thyroid carcinomas. Knockdown of TWIST1 byRNAinterference inATCcells reduced cell migration and invasion and increased sensitivity to apoptosis. The ectopic expression of TWIST1 in thyroid cells induced resistance to apoptosis and increased cell migration and invasion. Conclusions: TWIST1 plays a key role in determining malignant features of the anaplastic phenotype in vitro.

Original languageEnglish
JournalJournal of Clinical Endocrinology and Metabolism
Volume96
Issue number5
DOIs
Publication statusPublished - May 2011

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology
  • Biochemistry, medical
  • Endocrinology, Diabetes and Metabolism

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