Two γ-interferon-activation sites (GAS) on the promoter of the human intercellular adhesion molecule (ICAM-1) gene are required for induction of transcription by IFN-γ

Alessandra Tessitore, Laura Pastore, Alessandra Rispoli, Lucia Cilenti, Elena Toniato, Vincenzo Flati, Antonietta R. Farina, Luigi Frati, Alberto Gulino, Stefano Martinotti

Research output: Contribution to journalArticle

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Abstract

We describe the molecular features of the interferon (IFN)-γ-mediated transcription of the human intercellular adhesion molecule (ICAM-1) gene. We identified putative IFN-γ-activated sites (GAS) distributed throughout a large segment of the ICAM-1 promoter (4.0 kb region). Using computer-assisted search, these sequences were similar to potential IFN-γ responsive elements that have a core sequence 5'-TTNCNNNAA-3'. In this report we show that in the ICAM-1 promoter a GAS site is located at -115 from the translation initiation site, and binds with strong affinity to IFN-y-activated Signal Transducers and Activators of Transcription (STAT1) homodimers. The same sequence is responsible for the IFN-γ-mediated transcription of the ICAM-1 gene. Moreover, we present evidence that a more distal GAS element that maps at - 2787 from the translation initiation site, binds IFN-γ-activated STAT1 dimers with lower affinity. Multimeric copies of such GAS sequence inserted into a tkCAT minimal promoter can drive transcription, demonstrating that the -2787 bp GAS element has an independent functional activity upon binding of IFN-γ-activated STAT1 proteins as documented by in vitro binding assays. Furthermore, using recombinant ICAM-CAT mutants, we show that, in vivo, the - 2787 GAS, but not a mutagenized -2787 GAS site, when coupled to the more proximal -115 GAS element, has an additive effect in enhancing the IFN-γ- mediated transcription of ICAM-1 promoter. Nevertheless, using a recombinant construct bearing the wild type -2787 GAS element and a mutagenized -115 GAS element, we could not detect any transcription after transfection of U937 recipient cells, suggesting that the -2787 bp GAS element is not sufficient as such for gene activation, but can cooperate with its cognate proximal sequence to give full function to the ICAM-1 promoter during the IFN-γ response. Taken together these data provide evidence that two GAS sites are required for the full potential activity in the mechanism of ICAM-1 gene activation by IFN-γ.

Original languageEnglish
Pages (from-to)968-975
Number of pages8
JournalEuropean Journal of Biochemistry
Volume258
Issue number3
Publication statusPublished - Dec 15 1998

Fingerprint

Cell Adhesion Molecules
Intercellular Adhesion Molecule-1
Transcription
Interferons
Genes
Chemical activation
Transcriptional Activation
Bearings (structural)
STAT1 Transcription Factor
U937 Cells
Transducers
Dimers
Transfection
Assays

Keywords

  • Intercellular adhesion molecule 1
  • Interferon-γ
  • Interferon-γ-activation sites
  • Promoter
  • Signal transducer and activators of transcription

ASJC Scopus subject areas

  • Biochemistry

Cite this

Two γ-interferon-activation sites (GAS) on the promoter of the human intercellular adhesion molecule (ICAM-1) gene are required for induction of transcription by IFN-γ. / Tessitore, Alessandra; Pastore, Laura; Rispoli, Alessandra; Cilenti, Lucia; Toniato, Elena; Flati, Vincenzo; Farina, Antonietta R.; Frati, Luigi; Gulino, Alberto; Martinotti, Stefano.

In: European Journal of Biochemistry, Vol. 258, No. 3, 15.12.1998, p. 968-975.

Research output: Contribution to journalArticle

Tessitore, A, Pastore, L, Rispoli, A, Cilenti, L, Toniato, E, Flati, V, Farina, AR, Frati, L, Gulino, A & Martinotti, S 1998, 'Two γ-interferon-activation sites (GAS) on the promoter of the human intercellular adhesion molecule (ICAM-1) gene are required for induction of transcription by IFN-γ', European Journal of Biochemistry, vol. 258, no. 3, pp. 968-975.
Tessitore, Alessandra ; Pastore, Laura ; Rispoli, Alessandra ; Cilenti, Lucia ; Toniato, Elena ; Flati, Vincenzo ; Farina, Antonietta R. ; Frati, Luigi ; Gulino, Alberto ; Martinotti, Stefano. / Two γ-interferon-activation sites (GAS) on the promoter of the human intercellular adhesion molecule (ICAM-1) gene are required for induction of transcription by IFN-γ. In: European Journal of Biochemistry. 1998 ; Vol. 258, No. 3. pp. 968-975.
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abstract = "We describe the molecular features of the interferon (IFN)-γ-mediated transcription of the human intercellular adhesion molecule (ICAM-1) gene. We identified putative IFN-γ-activated sites (GAS) distributed throughout a large segment of the ICAM-1 promoter (4.0 kb region). Using computer-assisted search, these sequences were similar to potential IFN-γ responsive elements that have a core sequence 5'-TTNCNNNAA-3'. In this report we show that in the ICAM-1 promoter a GAS site is located at -115 from the translation initiation site, and binds with strong affinity to IFN-y-activated Signal Transducers and Activators of Transcription (STAT1) homodimers. The same sequence is responsible for the IFN-γ-mediated transcription of the ICAM-1 gene. Moreover, we present evidence that a more distal GAS element that maps at - 2787 from the translation initiation site, binds IFN-γ-activated STAT1 dimers with lower affinity. Multimeric copies of such GAS sequence inserted into a tkCAT minimal promoter can drive transcription, demonstrating that the -2787 bp GAS element has an independent functional activity upon binding of IFN-γ-activated STAT1 proteins as documented by in vitro binding assays. Furthermore, using recombinant ICAM-CAT mutants, we show that, in vivo, the - 2787 GAS, but not a mutagenized -2787 GAS site, when coupled to the more proximal -115 GAS element, has an additive effect in enhancing the IFN-γ- mediated transcription of ICAM-1 promoter. Nevertheless, using a recombinant construct bearing the wild type -2787 GAS element and a mutagenized -115 GAS element, we could not detect any transcription after transfection of U937 recipient cells, suggesting that the -2787 bp GAS element is not sufficient as such for gene activation, but can cooperate with its cognate proximal sequence to give full function to the ICAM-1 promoter during the IFN-γ response. Taken together these data provide evidence that two GAS sites are required for the full potential activity in the mechanism of ICAM-1 gene activation by IFN-γ.",
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AU - Rispoli, Alessandra

AU - Cilenti, Lucia

AU - Toniato, Elena

AU - Flati, Vincenzo

AU - Farina, Antonietta R.

AU - Frati, Luigi

AU - Gulino, Alberto

AU - Martinotti, Stefano

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N2 - We describe the molecular features of the interferon (IFN)-γ-mediated transcription of the human intercellular adhesion molecule (ICAM-1) gene. We identified putative IFN-γ-activated sites (GAS) distributed throughout a large segment of the ICAM-1 promoter (4.0 kb region). Using computer-assisted search, these sequences were similar to potential IFN-γ responsive elements that have a core sequence 5'-TTNCNNNAA-3'. In this report we show that in the ICAM-1 promoter a GAS site is located at -115 from the translation initiation site, and binds with strong affinity to IFN-y-activated Signal Transducers and Activators of Transcription (STAT1) homodimers. The same sequence is responsible for the IFN-γ-mediated transcription of the ICAM-1 gene. Moreover, we present evidence that a more distal GAS element that maps at - 2787 from the translation initiation site, binds IFN-γ-activated STAT1 dimers with lower affinity. Multimeric copies of such GAS sequence inserted into a tkCAT minimal promoter can drive transcription, demonstrating that the -2787 bp GAS element has an independent functional activity upon binding of IFN-γ-activated STAT1 proteins as documented by in vitro binding assays. Furthermore, using recombinant ICAM-CAT mutants, we show that, in vivo, the - 2787 GAS, but not a mutagenized -2787 GAS site, when coupled to the more proximal -115 GAS element, has an additive effect in enhancing the IFN-γ- mediated transcription of ICAM-1 promoter. Nevertheless, using a recombinant construct bearing the wild type -2787 GAS element and a mutagenized -115 GAS element, we could not detect any transcription after transfection of U937 recipient cells, suggesting that the -2787 bp GAS element is not sufficient as such for gene activation, but can cooperate with its cognate proximal sequence to give full function to the ICAM-1 promoter during the IFN-γ response. Taken together these data provide evidence that two GAS sites are required for the full potential activity in the mechanism of ICAM-1 gene activation by IFN-γ.

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