Two-dimensional protein map of human vastus lateralis muscle

Cecilia Gelfi, Sara De Palma, Paolo Cerretelli, Shagn Begum, Robin Wait

Research output: Contribution to journalArticlepeer-review

Abstract

Protein samples of human vastus lateralis muscle were analyzed by two-dimensional gel electrophoresis, using immobilized pH gradients encompassing several pH regions in the first dimension and sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the second dimension. More than 500 protein spots on each gel were detected by silver staining, of which 150 were excised, digested in-gel with trypsin and characterized by matrix assisted laser desorptioin/ionization-mass spectrometry and tandem electrospray mass spectrometry. Using these techniques, 124 spots including contractile proteins and metabolic enzymes were identified. This database should provide a valuable resource for the definition of the functional properties of muscle fibres and for assessment of the influence of e.g., hypoxia, ischaemia and ageing on protein level.

Original languageEnglish
Pages (from-to)286-295
Number of pages10
JournalElectrophoresis
Volume24
Issue number1-2
Publication statusPublished - Jan 2003

Keywords

  • Human skeletal muscle
  • Mass spectrometry
  • Proteomics
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Clinical Biochemistry

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