Two efficient polymeric chemical platforms for oligonucleotide microarray preparation

Clarissa Consolandi, Bianca Castiglioni, Roberta Bordoni, Elena Busti, Cristina Battaglia, Luigi Rossi Bernardi, Gianluca De Bellis

Research output: Contribution to journalArticlepeer-review

Abstract

In this report we describe two robust procedures for oligonucleotide microarray preparation based on polymeric coatings. The proposed chemical approaches include: 1) a glass functionalisation step with appropriate silanes (γ-aminopropyltriethoxysilane-APTES or 3-glycid-oxypropyltrimethoxysilane-GOPS), 2) a coating step using polymers (poly-L-Lysine or poly(acrylic acid-co-acrylamide) copolymer) covalently bound to the modified glass and 3) a surface activation step to allow for the attachment of amino-modified oligonucleotides. Results obtained using these chemistries in oligo microarray preparation show: 1) an overall high loading capacity and availability to hybridisation against targets, 2) a good uniformity, 3) resistance to consecutive probing/stripping cycles, 4) stability to thermal cycles, 5) effectiveness in hybridisation-mediated mutation detection procedures and 6) the possibility to perform enzymatic reactions, such as ligation.

Original languageEnglish
Pages (from-to)561-580
Number of pages20
JournalNucleosides, Nucleotides and Nucleic Acids
Volume21
Issue number8-9
DOIs
Publication statusPublished - 2002

Keywords

  • Amino-modified oligonucleotides
  • Mutation detection
  • Oligo-microarrays

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

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