Two efficient polymeric chemical platforms for oligonucleotide microarray preparation

Clarissa Consolandi, Bianca Castiglioni, Roberta Bordoni, Elena Busti, Cristina Battaglia, Luigi Rossi Bernardi, Gianluca De Bellis

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

In this report we describe two robust procedures for oligonucleotide microarray preparation based on polymeric coatings. The proposed chemical approaches include: 1) a glass functionalisation step with appropriate silanes (γ-aminopropyltriethoxysilane-APTES or 3-glycid-oxypropyltrimethoxysilane-GOPS), 2) a coating step using polymers (poly-L-Lysine or poly(acrylic acid-co-acrylamide) copolymer) covalently bound to the modified glass and 3) a surface activation step to allow for the attachment of amino-modified oligonucleotides. Results obtained using these chemistries in oligo microarray preparation show: 1) an overall high loading capacity and availability to hybridisation against targets, 2) a good uniformity, 3) resistance to consecutive probing/stripping cycles, 4) stability to thermal cycles, 5) effectiveness in hybridisation-mediated mutation detection procedures and 6) the possibility to perform enzymatic reactions, such as ligation.

Original languageEnglish
Pages (from-to)561-580
Number of pages20
JournalNucleosides, Nucleotides and Nucleic Acids
Volume21
Issue number8-9
DOIs
Publication statusPublished - 2002

Fingerprint

Microarrays
Oligonucleotide Array Sequence Analysis
Oligonucleotides
Glass
Silanes
Coatings
Lysine
Ligation
Polymers
Copolymers
Hot Temperature
Chemical activation
Availability
Mutation
amino-propyl-triethoxysilane
poly(acrylamide-co-acrylic acid)

Keywords

  • Amino-modified oligonucleotides
  • Mutation detection
  • Oligo-microarrays

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

Cite this

Two efficient polymeric chemical platforms for oligonucleotide microarray preparation. / Consolandi, Clarissa; Castiglioni, Bianca; Bordoni, Roberta; Busti, Elena; Battaglia, Cristina; Bernardi, Luigi Rossi; De Bellis, Gianluca.

In: Nucleosides, Nucleotides and Nucleic Acids, Vol. 21, No. 8-9, 2002, p. 561-580.

Research output: Contribution to journalArticle

Consolandi, C, Castiglioni, B, Bordoni, R, Busti, E, Battaglia, C, Bernardi, LR & De Bellis, G 2002, 'Two efficient polymeric chemical platforms for oligonucleotide microarray preparation', Nucleosides, Nucleotides and Nucleic Acids, vol. 21, no. 8-9, pp. 561-580. https://doi.org/10.1081/NCN-120015069
Consolandi, Clarissa ; Castiglioni, Bianca ; Bordoni, Roberta ; Busti, Elena ; Battaglia, Cristina ; Bernardi, Luigi Rossi ; De Bellis, Gianluca. / Two efficient polymeric chemical platforms for oligonucleotide microarray preparation. In: Nucleosides, Nucleotides and Nucleic Acids. 2002 ; Vol. 21, No. 8-9. pp. 561-580.
@article{1d3c8ec8c476439297e036eec3f2f616,
title = "Two efficient polymeric chemical platforms for oligonucleotide microarray preparation",
abstract = "In this report we describe two robust procedures for oligonucleotide microarray preparation based on polymeric coatings. The proposed chemical approaches include: 1) a glass functionalisation step with appropriate silanes (γ-aminopropyltriethoxysilane-APTES or 3-glycid-oxypropyltrimethoxysilane-GOPS), 2) a coating step using polymers (poly-L-Lysine or poly(acrylic acid-co-acrylamide) copolymer) covalently bound to the modified glass and 3) a surface activation step to allow for the attachment of amino-modified oligonucleotides. Results obtained using these chemistries in oligo microarray preparation show: 1) an overall high loading capacity and availability to hybridisation against targets, 2) a good uniformity, 3) resistance to consecutive probing/stripping cycles, 4) stability to thermal cycles, 5) effectiveness in hybridisation-mediated mutation detection procedures and 6) the possibility to perform enzymatic reactions, such as ligation.",
keywords = "Amino-modified oligonucleotides, Mutation detection, Oligo-microarrays",
author = "Clarissa Consolandi and Bianca Castiglioni and Roberta Bordoni and Elena Busti and Cristina Battaglia and Bernardi, {Luigi Rossi} and {De Bellis}, Gianluca",
year = "2002",
doi = "10.1081/NCN-120015069",
language = "English",
volume = "21",
pages = "561--580",
journal = "Nucleosides and Nucleotides",
issn = "0732-8311",
publisher = "Taylor and Francis Ltd.",
number = "8-9",

}

TY - JOUR

T1 - Two efficient polymeric chemical platforms for oligonucleotide microarray preparation

AU - Consolandi, Clarissa

AU - Castiglioni, Bianca

AU - Bordoni, Roberta

AU - Busti, Elena

AU - Battaglia, Cristina

AU - Bernardi, Luigi Rossi

AU - De Bellis, Gianluca

PY - 2002

Y1 - 2002

N2 - In this report we describe two robust procedures for oligonucleotide microarray preparation based on polymeric coatings. The proposed chemical approaches include: 1) a glass functionalisation step with appropriate silanes (γ-aminopropyltriethoxysilane-APTES or 3-glycid-oxypropyltrimethoxysilane-GOPS), 2) a coating step using polymers (poly-L-Lysine or poly(acrylic acid-co-acrylamide) copolymer) covalently bound to the modified glass and 3) a surface activation step to allow for the attachment of amino-modified oligonucleotides. Results obtained using these chemistries in oligo microarray preparation show: 1) an overall high loading capacity and availability to hybridisation against targets, 2) a good uniformity, 3) resistance to consecutive probing/stripping cycles, 4) stability to thermal cycles, 5) effectiveness in hybridisation-mediated mutation detection procedures and 6) the possibility to perform enzymatic reactions, such as ligation.

AB - In this report we describe two robust procedures for oligonucleotide microarray preparation based on polymeric coatings. The proposed chemical approaches include: 1) a glass functionalisation step with appropriate silanes (γ-aminopropyltriethoxysilane-APTES or 3-glycid-oxypropyltrimethoxysilane-GOPS), 2) a coating step using polymers (poly-L-Lysine or poly(acrylic acid-co-acrylamide) copolymer) covalently bound to the modified glass and 3) a surface activation step to allow for the attachment of amino-modified oligonucleotides. Results obtained using these chemistries in oligo microarray preparation show: 1) an overall high loading capacity and availability to hybridisation against targets, 2) a good uniformity, 3) resistance to consecutive probing/stripping cycles, 4) stability to thermal cycles, 5) effectiveness in hybridisation-mediated mutation detection procedures and 6) the possibility to perform enzymatic reactions, such as ligation.

KW - Amino-modified oligonucleotides

KW - Mutation detection

KW - Oligo-microarrays

UR - http://www.scopus.com/inward/record.url?scp=1842845771&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1842845771&partnerID=8YFLogxK

U2 - 10.1081/NCN-120015069

DO - 10.1081/NCN-120015069

M3 - Article

VL - 21

SP - 561

EP - 580

JO - Nucleosides and Nucleotides

JF - Nucleosides and Nucleotides

SN - 0732-8311

IS - 8-9

ER -