Two ENU-induced mutations in Rasgrf1 and early mouse growth retardation

Steven J. Clapcott, Josephine Peters, Paul C. Orban, Riccardo Brambilla, Chris F. Graham

Research output: Contribution to journalArticle

Abstract

When paternally transmitted, two independent ENU-induced mutations showed reduced whole body wet weight soon after birth. The mutations were mapped to Chromosome 9 (Chr 9) between the markers DgMit208 and DgMit215. Their map position and imprinted status suggested that they might alter RAS protein-specific guanine nucleotide releasing factor 1 expression. Both mutations introduced premature chain termination codons into the coding sequence of Rasgrf1, and no Ras-GRF1 protein was detected in the brain. The GENA53 line had a C to T transition at nucleotide 2137, and the line GENA37 had a T to A transversion at nucleotide 3552 of the cDNA sequence. Mutant mice had near normal body weight at birth, but their weight started to lag behind that of wild-type littermates during the first week, and they were about 15% lighter as adults.

Original languageEnglish
Pages (from-to)495-505
Number of pages11
JournalMammalian Genome
Volume14
Issue number8
DOIs
Publication statusPublished - Aug 1 2003

Fingerprint

ras-GRF1
Mutation
Nucleotides
Growth
Guanine Nucleotide Exchange Factors
ras Proteins
Ideal Body Weight
Chromosomes, Human, Pair 9
Nonsense Codon
Birth Weight
Complementary DNA
Body Weight
Parturition
Brain
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Clapcott, S. J., Peters, J., Orban, P. C., Brambilla, R., & Graham, C. F. (2003). Two ENU-induced mutations in Rasgrf1 and early mouse growth retardation. Mammalian Genome, 14(8), 495-505. https://doi.org/10.1007/s00335-002-2258-4

Two ENU-induced mutations in Rasgrf1 and early mouse growth retardation. / Clapcott, Steven J.; Peters, Josephine; Orban, Paul C.; Brambilla, Riccardo; Graham, Chris F.

In: Mammalian Genome, Vol. 14, No. 8, 01.08.2003, p. 495-505.

Research output: Contribution to journalArticle

Clapcott, SJ, Peters, J, Orban, PC, Brambilla, R & Graham, CF 2003, 'Two ENU-induced mutations in Rasgrf1 and early mouse growth retardation', Mammalian Genome, vol. 14, no. 8, pp. 495-505. https://doi.org/10.1007/s00335-002-2258-4
Clapcott SJ, Peters J, Orban PC, Brambilla R, Graham CF. Two ENU-induced mutations in Rasgrf1 and early mouse growth retardation. Mammalian Genome. 2003 Aug 1;14(8):495-505. https://doi.org/10.1007/s00335-002-2258-4
Clapcott, Steven J. ; Peters, Josephine ; Orban, Paul C. ; Brambilla, Riccardo ; Graham, Chris F. / Two ENU-induced mutations in Rasgrf1 and early mouse growth retardation. In: Mammalian Genome. 2003 ; Vol. 14, No. 8. pp. 495-505.
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