Two functionally distinct subsets of mast cells discriminated by IL-2-independent CD25 activities

Lorenzo Deho, Cristina Leoni, Tess M. Brodie, Sara Montagner, Marco De Simone, Sara Polletti, Iros Barozzi, Gioacchino Natoli, Silvia Monticelli

Research output: Contribution to journalArticle

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Abstract

We identified two mast cell subsets characterized by the differential expression of surface CD25 (IL-2Ra) and by different abilities to produce cytokines and to proliferate, both in vitro and in vivo. CD25 can be expressed on the surface of immune cells in the absence of the other chains of the IL-2R, which are indispensable for IL-2 signaling. We show that functional differences between the two mast cell populations were dependent on CD25 itself, which directly modulated proliferation and cytokine responses. These effects were completely independent from IL-2 or the expression of the other chains of the high-affinity IL-2R, indicating an autonomous and previously unappreciated role for CD25 in regulating cell functions. Cells genetically ablated for CD25 completely recapitulated the CD25-negative phenotype and never acquired the properties characteristic of CD25-positive mast cells. Finally, adoptive transfer experiments in the mouse demonstrated a different impact of these populations in models of anaphylaxis and contact sensitivity. Our findings indicate a general role for CD25 in contexts where IL-2 signaling is not involved, and may have important implications for all mast cell-related diseases, as well as in all cell types expressing CD25 independently of its IL-2- related functions. Copyright

Original languageEnglish
Pages (from-to)2196-2206
Number of pages11
JournalJournal of Immunology
Volume193
Issue number5
DOIs
Publication statusPublished - Sep 1 2014

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Mast Cells
Interleukin-2
Cytokines
Mastocytosis
Adoptive Transfer
Contact Dermatitis
Anaphylaxis
Population
Phenotype

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Deho, L., Leoni, C., Brodie, T. M., Montagner, S., De Simone, M., Polletti, S., ... Monticelli, S. (2014). Two functionally distinct subsets of mast cells discriminated by IL-2-independent CD25 activities. Journal of Immunology, 193(5), 2196-2206. https://doi.org/10.4049/jimmunol.1400516

Two functionally distinct subsets of mast cells discriminated by IL-2-independent CD25 activities. / Deho, Lorenzo; Leoni, Cristina; Brodie, Tess M.; Montagner, Sara; De Simone, Marco; Polletti, Sara; Barozzi, Iros; Natoli, Gioacchino; Monticelli, Silvia.

In: Journal of Immunology, Vol. 193, No. 5, 01.09.2014, p. 2196-2206.

Research output: Contribution to journalArticle

Deho, L, Leoni, C, Brodie, TM, Montagner, S, De Simone, M, Polletti, S, Barozzi, I, Natoli, G & Monticelli, S 2014, 'Two functionally distinct subsets of mast cells discriminated by IL-2-independent CD25 activities', Journal of Immunology, vol. 193, no. 5, pp. 2196-2206. https://doi.org/10.4049/jimmunol.1400516
Deho, Lorenzo ; Leoni, Cristina ; Brodie, Tess M. ; Montagner, Sara ; De Simone, Marco ; Polletti, Sara ; Barozzi, Iros ; Natoli, Gioacchino ; Monticelli, Silvia. / Two functionally distinct subsets of mast cells discriminated by IL-2-independent CD25 activities. In: Journal of Immunology. 2014 ; Vol. 193, No. 5. pp. 2196-2206.
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