Abstract
We have previously reported the existence of 2 forms of mRNA for the human muscle acetylcholine receptor (AChR) α-subunit, thought to be generated by alternate splicing of a primary transcript and to encode 2 α-subunit protein isoforms [1]. The 2 predicted α-subunit isoforms, differing by the insertion of 25 amino acids at position 58 59, have been synthesized from cRNA transcripts using rabbit reticulocyte lysates: these protein isoforms could be differentiated by immunoprecipitation using antibodies raised against synthetic peptides. The antibodies were used to demonstrate translation of both AChR α-subunit isoforms in the rhabdomyosarcoma (muscle) cell line TE671, in an approximate 1:1 ratio.
Original language | English |
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Pages (from-to) | 116-118 |
Number of pages | 3 |
Journal | FEBS Letters |
Volume | 295 |
Issue number | 1-3 |
DOIs | |
Publication status | Published - Dec 16 1991 |
Keywords
- AChR: α-Subunit
- Isoform
- TE671 cell
- Translation
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology