Two isoforms of the muscle acetylcholine receptor α-subunit are translated in the human cell line TE671

A. Morris, D. Beeson, L. Jacobson, F. Baggi, A. Vincent, J. Newsom-Davis

Research output: Contribution to journalArticlepeer-review

Abstract

We have previously reported the existence of 2 forms of mRNA for the human muscle acetylcholine receptor (AChR) α-subunit, thought to be generated by alternate splicing of a primary transcript and to encode 2 α-subunit protein isoforms [1]. The 2 predicted α-subunit isoforms, differing by the insertion of 25 amino acids at position 58 59, have been synthesized from cRNA transcripts using rabbit reticulocyte lysates: these protein isoforms could be differentiated by immunoprecipitation using antibodies raised against synthetic peptides. The antibodies were used to demonstrate translation of both AChR α-subunit isoforms in the rhabdomyosarcoma (muscle) cell line TE671, in an approximate 1:1 ratio.

Original languageEnglish
Pages (from-to)116-118
Number of pages3
JournalFEBS Letters
Volume295
Issue number1-3
DOIs
Publication statusPublished - Dec 16 1991

Keywords

  • AChR: α-Subunit
  • Isoform
  • TE671 cell
  • Translation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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