Two mutations in a conserved structural motif in the insulin receptor inhibit normal folding and intracellular transport of the receptor

Efrat Wertheimer, Fabrizio Barbetti, Michele Muggeo, Jesse Roth, Simeon I. Taylor

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Abstract

Insulin initiates its biological response by binding to the extracellular domain of the insulin receptor. The N-terminal half of the α-subunit contains several repeats of a loosely conserved motif consisting of a central glycine plus several hydrophobic amino acid residues upstream from the glycine, Hyφ-Xaa-Xaa-Hyφ-Xaa-Hyφ-Hyφ-Xaa-Gly (where Hyφ represents a hydrophobic amino acid residue). This structural motif has been proposed to be important in determining the three-dimensional structure of the insulin binding domain. We have identified two naturally occurring mutant alleles of the insulin receptor gene in an insulin-resistant patient, substitution of Ala for Val28 and Arg for Gly366. The mutations alter conserved amino acid residues in two distinct repeats of the structural motif described above. When mutant cDNAs were expressed in NIH-3T3 cells, both mutations severely impaired proteolytic processing of the proreceptor to mature α- and β-subunits. Transport of mutant receptors to the plasma membrane was also impaired. However, the minority (28 and Arg366 mutations prevent normal folding of the insulin receptor α-subunit, thereby inhibiting post-translational processing and intracellular transport of the mutant receptors.

Original languageEnglish
Pages (from-to)7587-7592
Number of pages6
JournalJournal of Biological Chemistry
Volume269
Issue number10
Publication statusPublished - Mar 11 1994

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Insulin Receptor
Insulin
Amino Acids
Glycine
Mutation
NIH 3T3 Cells
Cell membranes
Processing
Substitution reactions
Complementary DNA
Genes
Alleles
Cell Membrane

ASJC Scopus subject areas

  • Biochemistry

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Two mutations in a conserved structural motif in the insulin receptor inhibit normal folding and intracellular transport of the receptor. / Wertheimer, Efrat; Barbetti, Fabrizio; Muggeo, Michele; Roth, Jesse; Taylor, Simeon I.

In: Journal of Biological Chemistry, Vol. 269, No. 10, 11.03.1994, p. 7587-7592.

Research output: Contribution to journalArticle

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