Two new formylated peptides able to activate chemotaxis and respiratory burst selectively as tools for studying human neutrophil responses

M. Enrica Ferretti, Susanna Spisani, M. Cristina Pareschi, Marco Buzzi, Roberta Cavallaro, Serena Traniello, Eva Reali, Ines Torrini, Mario Paglialunga Paradisi, Giampiero Pagani Zecanni, Carla Biondi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Two new For-Met-Leu-Phe-OH (FMLP) methyl ester analogues, For-Thp-Leu-Ain-OMe [Thp1, Ain3] and For-Met-Δ(z)Leu-Phe-OMe [Δ(z)Leu2], able to activate selectively chemotaxis and superoxide anion (O2 -) release, respectively modulate intracellular cyclic AMP (cAMP) levels in different ways. FMLP and [Δ(z)Leu2] enhance human neutrophil cAMP levels per se, and this effect is potentiated by Ro 201724, a non-xanthinic phosphodiesterase (PDE) inhibitor, whereas it is counteracted by 3-isobutyl-1-methyl-xanthine (IBMX), a blocker of both phosphodiesterase and adenosine receptors. In contrast, [Thp1, Ain3] is ineffective. However, no formylated peptides influence cAMP phosphodiesterase activity. Neutrophil preincubation with Ro 201724 or IBMX drastically reduces chemotaxis and superoxide anion (O2 -) production triggered by peptides. Our results suggest that: (1) peptide-induced cAMP increase is probably indirect, and due mainly to the action on adenosine-sensitive adenylate cyclase; (2) formylated peptide, endowed solely with chemotactic activity is unable to increase neutrophil cAMP concentration; (3) cAMP elevation may represent a feed-back mechanism to inhibit the physiological responses induced by formylated peptides.

Original languageEnglish
Pages (from-to)91-101
Number of pages11
JournalCellular Signalling
Volume6
Issue number1
DOIs
Publication statusPublished - 1994

Fingerprint

Respiratory Burst
Chemotaxis
Cyclic AMP
Neutrophils
Peptides
4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone
methionyl-leucyl-phenylalanine
Xanthine
Phosphoric Diester Hydrolases
Superoxides
Purinergic P1 Receptors
Phosphodiesterase Inhibitors
Adenylyl Cyclases
Adenosine
Esters

Keywords

  • cAMP system
  • chemotaxis
  • formylated peptides
  • neutrophils
  • O release
  • phosphodiesterase inhibitors

ASJC Scopus subject areas

  • Cell Biology

Cite this

Enrica Ferretti, M., Spisani, S., Cristina Pareschi, M., Buzzi, M., Cavallaro, R., Traniello, S., ... Biondi, C. (1994). Two new formylated peptides able to activate chemotaxis and respiratory burst selectively as tools for studying human neutrophil responses. Cellular Signalling, 6(1), 91-101. https://doi.org/10.1016/0898-6568(94)90064-7

Two new formylated peptides able to activate chemotaxis and respiratory burst selectively as tools for studying human neutrophil responses. / Enrica Ferretti, M.; Spisani, Susanna; Cristina Pareschi, M.; Buzzi, Marco; Cavallaro, Roberta; Traniello, Serena; Reali, Eva; Torrini, Ines; Paradisi, Mario Paglialunga; Zecanni, Giampiero Pagani; Biondi, Carla.

In: Cellular Signalling, Vol. 6, No. 1, 1994, p. 91-101.

Research output: Contribution to journalArticle

Enrica Ferretti, M, Spisani, S, Cristina Pareschi, M, Buzzi, M, Cavallaro, R, Traniello, S, Reali, E, Torrini, I, Paradisi, MP, Zecanni, GP & Biondi, C 1994, 'Two new formylated peptides able to activate chemotaxis and respiratory burst selectively as tools for studying human neutrophil responses', Cellular Signalling, vol. 6, no. 1, pp. 91-101. https://doi.org/10.1016/0898-6568(94)90064-7
Enrica Ferretti, M. ; Spisani, Susanna ; Cristina Pareschi, M. ; Buzzi, Marco ; Cavallaro, Roberta ; Traniello, Serena ; Reali, Eva ; Torrini, Ines ; Paradisi, Mario Paglialunga ; Zecanni, Giampiero Pagani ; Biondi, Carla. / Two new formylated peptides able to activate chemotaxis and respiratory burst selectively as tools for studying human neutrophil responses. In: Cellular Signalling. 1994 ; Vol. 6, No. 1. pp. 91-101.
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