Two-photon activation and excitation properties of PA-GFP in the 720-920-nm region

Marc Schneider, Sara Barozzi, Maria Testa, Mario Faretta, Alberto Diaspro

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

This report covers the two-photon activation and excitation properties of the PA-GFP, a photoactivatable variant of the Aequorea victoria green fluorescent protein in the spectral region from 720 to 920 nm. It is known from this special form of the molecule that it has an increased level of fluorescence emission when excited at 488 nm after irradiation at λ 413 nm, under single-photon excitation conditions. Here, we show that upon two-photon irradiation, PA-GFP yields activation in the spectral region from 720 to 840 nm. After photoactivation, the excitation spectrum shifts maintaining the very same emission spectrum of the single-photon case forthe native and photoactivated protein. Additionally, when comparing the conventional photoactivation at λ = 405 nm with a two-photon one, a sharper and better controllable three-dimensional volume of activation is obtained.

Original languageEnglish
Pages (from-to)1346-1352
Number of pages7
JournalBiophysical Journal
Volume89
Issue number2
DOIs
Publication statusPublished - Aug 2005

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Photons
Fluorescence
Proteins

ASJC Scopus subject areas

  • Biophysics

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Two-photon activation and excitation properties of PA-GFP in the 720-920-nm region. / Schneider, Marc; Barozzi, Sara; Testa, Maria; Faretta, Mario; Diaspro, Alberto.

In: Biophysical Journal, Vol. 89, No. 2, 08.2005, p. 1346-1352.

Research output: Contribution to journalArticle

Schneider, Marc ; Barozzi, Sara ; Testa, Maria ; Faretta, Mario ; Diaspro, Alberto. / Two-photon activation and excitation properties of PA-GFP in the 720-920-nm region. In: Biophysical Journal. 2005 ; Vol. 89, No. 2. pp. 1346-1352.
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