Two-photon excitation and confocal microscopy investigation of nanostructured polyelectrolyte shells

Alberto Diaspro, Ornella Cavalleri, Daniela Silvano, Silke Krol, Alessandra Gliozzi

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Two photon excitation (TPE) and confocal laser scanning microscopy (CLSM) allow obtaining structural and functional information at sub-micron resolution of three-dimensional specimens. We are interested in the relationship between the architectural motifs and functional properties of organized assemblies of nanostructured polyelectrolyte shells. Stepwise adsorption of oppositely charged polyelectrolytes onto micrometer sized organic and inorganic cores followed by core dissolution results in the formation of hollow polyelectrolyte shells, named nanocapsules. Nanocapsules can be used as protected environments for guest molecules allowing controlled release or entrance of substances. They are of both biological and medical interest since can be used for the controlled release and targeting of drags as well as for the protection of enzymes and proteins. The structure and the permeability properties of the shells in hydrated state were investigated by a TPE architecture based on a compact CLSM, Nikon PCM2000, in which the ability to operate as a standard CLSM was preserved. Imaging was also probed by scanning force microscopy measurements on dried shells. We also plan to check other strategies for core removal and to perform confined experiments into the nanocaspules, including photopatterning and photobleaching ones.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsA. Periasamy, P.T.C. So
Number of pages6
Publication statusPublished - 2002
EventMultiphoton Microscopy in the Biomedical Sciences II - San Jose, CA, United States
Duration: Jan 20 2002Jan 22 2002


OtherMultiphoton Microscopy in the Biomedical Sciences II
Country/TerritoryUnited States
CitySan Jose, CA


  • Confocal Microscopy
  • Nanocapsule
  • Photodamaging
  • Photopatterning
  • Polyelectrolyte
  • Two-photon excitation microscopy

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics


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