Two regions of deletion in 9p22∼p24 in neuroblastoma are frequently observed in favorable tumors

Lucia Giordani, Achille Iolascon, Veronica Servedio, Katia Mazzocco, Luca Longo, Gian Paolo Tonini

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Neuroblastoma is a tumor of infancy that presents several chromosomal abnormalities. Nonrandom deletion of chromosome arm 9p has been identified in primary neuroblastoma suggesting the presence of a tumor suppressor gene located on this chromosome. In previous work, we showed that CDKN2A and CDKN2B genes, mapped at 9p21, were not deleted in neuroblastoma cells. In the present article, we refine the deleted region of 9p using polymerase chain reaction-based analysis of highly polymorphic simple sequence repeats and a two color fluorescence in situ hybridization technique on interphase nuclei. We analyzed 71 primary tumors of patients at the onset of the disease. We found loss of heterozygosity (LOH) in 16 of 71 (23%) cases; the frequency of LOH for 9p was higher (28%) in favorable stages 1, 2, and 4s than in unfavorable stages 3 and 4 (14%). Our results identify two regions of frequent allelic loss: the first at the locus D9S1849 and the second at the locus D9S157. These regions appear to be distant from CDKN2A and CDKN2B loci suggesting that other genes may be involved in 9p deletion. Finally, our data show that 9p deletion is more frequent in tumors of patients with a favorable prognosis, indicating that deleted genes may not be crucial for tumor progression.

Original languageEnglish
Pages (from-to)42-47
Number of pages6
JournalCancer Genetics and Cytogenetics
Volume135
Issue number1
DOIs
Publication statusPublished - 2002

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Neuroblastoma
Loss of Heterozygosity
Neoplasms
p16 Genes
Chromosome Deletion
Interphase
Tumor Suppressor Genes
Fluorescence In Situ Hybridization
Chromosome Aberrations
Microsatellite Repeats
Genes
Color
Chromosomes
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Two regions of deletion in 9p22∼p24 in neuroblastoma are frequently observed in favorable tumors. / Giordani, Lucia; Iolascon, Achille; Servedio, Veronica; Mazzocco, Katia; Longo, Luca; Tonini, Gian Paolo.

In: Cancer Genetics and Cytogenetics, Vol. 135, No. 1, 2002, p. 42-47.

Research output: Contribution to journalArticle

Giordani, Lucia ; Iolascon, Achille ; Servedio, Veronica ; Mazzocco, Katia ; Longo, Luca ; Tonini, Gian Paolo. / Two regions of deletion in 9p22∼p24 in neuroblastoma are frequently observed in favorable tumors. In: Cancer Genetics and Cytogenetics. 2002 ; Vol. 135, No. 1. pp. 42-47.
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AU - Longo, Luca

AU - Tonini, Gian Paolo

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