Abstract
Purpose: The purpose of this study was to assess two-step in vivo tumor targeting by specific biotin-conjugated antibodies and ultrasmall superparamagnetic iron oxide (USPIO)-anti-biotin nanoparticles as contrast agents for magnetic resonance imaging (MRI) at 1.5 T. Procedures: D430B human lymphoma cells, expressing the CD70 surface antigen, were injected either s.c. or i.v. to induce pseudo-metastases in NOD/SCID mice. Thirty micrograms of biotin-conjugated monoclonal anti-CD70 was injected i.v., followed 4 h later by 8 μmol Fe/Kg USPIO-anti-biotin. After 24 h, MRI was performed on T2* and b-FFE sequences. Signal intensity (SI) was calculated before and after USPIO-anti-biotin administration. Results: Subcutaneous xenografts showed a dishomogeneous 30% decrease in SI on T2* with anti-CD70+USPIO-anti-biotin treatment. Pseudo-metastatic xenografts showed a slight reduction in SI on T2*, but a 60% decrease in SI on b-FFE-weighted sequences. Prussian blue staining confirmed the presence of iron nanoparticles in the excised tumors. Conclusion: MRI at 1.5 T can detect tumors by a two-step in vivo biotin-based protocol, which may allow the targeting of any cell surface antigen.
| Original language | English |
|---|---|
| Pages (from-to) | 305-315 |
| Number of pages | 11 |
| Journal | Molecular Imaging and Biology |
| Volume | 12 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jun 2010 |
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Keywords
- Antibody
- In vivo small animal MRI
- Iron oxide particles
- Magnetic resonance imaging
- Targeted contrast agent
ASJC Scopus subject areas
- Cancer Research
- Oncology
- Radiology Nuclear Medicine and imaging
Cite this
Two-step in vivo tumor targeting by biotin- Conjugated antibodies and superparamagnetic nanoparticles assessed by magnetic resonance imaging at 1.5 T. / Baio, Gabriella; Fabbi, Marina; Salvi, Sandra; De Totero, Daniela; Truini, Mauro; Ferrini, Silvano; Neumaier, Carlo Emanuele.
In: Molecular Imaging and Biology, Vol. 12, No. 3, 06.2010, p. 305-315.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Two-step in vivo tumor targeting by biotin- Conjugated antibodies and superparamagnetic nanoparticles assessed by magnetic resonance imaging at 1.5 T
AU - Baio, Gabriella
AU - Fabbi, Marina
AU - Salvi, Sandra
AU - De Totero, Daniela
AU - Truini, Mauro
AU - Ferrini, Silvano
AU - Neumaier, Carlo Emanuele
PY - 2010/6
Y1 - 2010/6
N2 - Purpose: The purpose of this study was to assess two-step in vivo tumor targeting by specific biotin-conjugated antibodies and ultrasmall superparamagnetic iron oxide (USPIO)-anti-biotin nanoparticles as contrast agents for magnetic resonance imaging (MRI) at 1.5 T. Procedures: D430B human lymphoma cells, expressing the CD70 surface antigen, were injected either s.c. or i.v. to induce pseudo-metastases in NOD/SCID mice. Thirty micrograms of biotin-conjugated monoclonal anti-CD70 was injected i.v., followed 4 h later by 8 μmol Fe/Kg USPIO-anti-biotin. After 24 h, MRI was performed on T2* and b-FFE sequences. Signal intensity (SI) was calculated before and after USPIO-anti-biotin administration. Results: Subcutaneous xenografts showed a dishomogeneous 30% decrease in SI on T2* with anti-CD70+USPIO-anti-biotin treatment. Pseudo-metastatic xenografts showed a slight reduction in SI on T2*, but a 60% decrease in SI on b-FFE-weighted sequences. Prussian blue staining confirmed the presence of iron nanoparticles in the excised tumors. Conclusion: MRI at 1.5 T can detect tumors by a two-step in vivo biotin-based protocol, which may allow the targeting of any cell surface antigen.
AB - Purpose: The purpose of this study was to assess two-step in vivo tumor targeting by specific biotin-conjugated antibodies and ultrasmall superparamagnetic iron oxide (USPIO)-anti-biotin nanoparticles as contrast agents for magnetic resonance imaging (MRI) at 1.5 T. Procedures: D430B human lymphoma cells, expressing the CD70 surface antigen, were injected either s.c. or i.v. to induce pseudo-metastases in NOD/SCID mice. Thirty micrograms of biotin-conjugated monoclonal anti-CD70 was injected i.v., followed 4 h later by 8 μmol Fe/Kg USPIO-anti-biotin. After 24 h, MRI was performed on T2* and b-FFE sequences. Signal intensity (SI) was calculated before and after USPIO-anti-biotin administration. Results: Subcutaneous xenografts showed a dishomogeneous 30% decrease in SI on T2* with anti-CD70+USPIO-anti-biotin treatment. Pseudo-metastatic xenografts showed a slight reduction in SI on T2*, but a 60% decrease in SI on b-FFE-weighted sequences. Prussian blue staining confirmed the presence of iron nanoparticles in the excised tumors. Conclusion: MRI at 1.5 T can detect tumors by a two-step in vivo biotin-based protocol, which may allow the targeting of any cell surface antigen.
KW - Antibody
KW - In vivo small animal MRI
KW - Iron oxide particles
KW - Magnetic resonance imaging
KW - Targeted contrast agent
UR - http://www.scopus.com/inward/record.url?scp=77956707999&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77956707999&partnerID=8YFLogxK
U2 - 10.1007/s11307-009-0264-6
DO - 10.1007/s11307-009-0264-6
M3 - Article
C2 - 19806404
AN - SCOPUS:77956707999
VL - 12
SP - 305
EP - 315
JO - Molecular Imaging and Biology
JF - Molecular Imaging and Biology
SN - 1536-1632
IS - 3
ER -