TxA2 production by human arteries and veins

G. G. Neri Serneri, R. Abbate, G. F. Gensini, A. Panetta, G. C. Casolo, M. Carini

Research output: Contribution to journalArticle

Abstract

Human arterial and venous segments from patients under-going operations when incubated in Tris buffer both alone and with arachidonic acid were able to produce thromboxane B2 (assessed by radioimmunoassay). Thromoboxane B2 (TxB2) production was progressive in time (till 40 min.) and was enhanced by the addition of 1mM norepinephrien. Contamination of tissues by platelet was checked and platelets did not contribute to thromboxane formation. The investigation of the conversions of 1-14C arachidonic acid by vascular tissue indicated that human vascular tissues produce the metabolites of the cyclooxygenase dependent pathway and that prostacyclin is the main metabolite with a PGI2/TxA2 ratio of 4:1. The arterial wall was found to posses an active lipoxygenase dependent pathway. Thromboxane production by intimal cells was neglible and the main source of thromboxane was the media. The production of thromboxane did not change in relation to age, but arterial segments from men produced significantly larger amounts of thromboxane than those from women.

Original languageEnglish
Pages (from-to)753-766
Number of pages14
JournalProstaglandins
Volume25
Issue number6
DOIs
Publication statusPublished - 1983

Fingerprint

Thromboxanes
Veins
Arteries
Epoprostenol
Tissue
Metabolites
Platelets
Arachidonic Acid
Blood Vessels
Blood Platelets
Tunica Intima
Thromboxane B2
Tromethamine
Lipoxygenase
Prostaglandin-Endoperoxide Synthases
Radioimmunoassay
Contamination

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Neri Serneri, G. G., Abbate, R., Gensini, G. F., Panetta, A., Casolo, G. C., & Carini, M. (1983). TxA2 production by human arteries and veins. Prostaglandins, 25(6), 753-766. https://doi.org/10.1016/0090-6980(83)90001-1

TxA2 production by human arteries and veins. / Neri Serneri, G. G.; Abbate, R.; Gensini, G. F.; Panetta, A.; Casolo, G. C.; Carini, M.

In: Prostaglandins, Vol. 25, No. 6, 1983, p. 753-766.

Research output: Contribution to journalArticle

Neri Serneri, GG, Abbate, R, Gensini, GF, Panetta, A, Casolo, GC & Carini, M 1983, 'TxA2 production by human arteries and veins', Prostaglandins, vol. 25, no. 6, pp. 753-766. https://doi.org/10.1016/0090-6980(83)90001-1
Neri Serneri GG, Abbate R, Gensini GF, Panetta A, Casolo GC, Carini M. TxA2 production by human arteries and veins. Prostaglandins. 1983;25(6):753-766. https://doi.org/10.1016/0090-6980(83)90001-1
Neri Serneri, G. G. ; Abbate, R. ; Gensini, G. F. ; Panetta, A. ; Casolo, G. C. ; Carini, M. / TxA2 production by human arteries and veins. In: Prostaglandins. 1983 ; Vol. 25, No. 6. pp. 753-766.
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