Type-1 cannabinoid receptors colocalize with caveolin-1 in neuronal cells

Monica Bari, Sergio Oddi, Chiara De Simone, Paola Spagnolo, Valeria Gasperi, Natalia Battista, Diego Centonze, Mauro Maccarrone

Research output: Contribution to journalArticlepeer-review

Abstract

Type-1 (CB1) and type-2 (CB2) cannabinoid receptors belong to the rhodopsin family of G protein-coupled receptors, and are activated by endogenous lipids termed "endocannabinoids". Recent reports have demonstrated that CB1R, unlike CB2R and other receptors and metabolic enzymes of endocannabinoids, functions in the context of lipid rafts, i.e. plasma membrane microdomains which may be important in modulating signal transduction. Here, we present novel data based on cell subfractionation, immunoprecipitation and confocal microscopy studies, that show that in C6 cells CB1R co-localizes almost entirely with caveolin-1. We also show that trafficking of CB1R in response to the raft disruptor methyl-β-cyclodextrin (MCD) is superimposable on that of caveolin-1, and that MCD treatment increases the accessibility of CB1R to its specific antibodies. These findings may be relevant for the manifold CB1R-dependent activities of endocannabinoids, like the regulation of apoptosis and of neurodegenerative diseases.

Original languageEnglish
Pages (from-to)45-50
Number of pages6
JournalNeuropharmacology
Volume54
Issue number1
DOIs
Publication statusPublished - Jan 2008

Keywords

  • Caveolin
  • Cholesterol
  • Endocannabinoids
  • G protein-coupled receptors
  • Lipid rafts
  • Neurodegeneration
  • Signal transduction
  • Trafficking

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Drug Discovery
  • Pharmacology

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