Type-1 Cannabinoid receptors reduce membrane fluidity of capacitated boar sperm by impairing their activation by bicarbonate

Barbara Barboni, Nicola Bernabò, Paola Palestini, Laura Botto, Maria Gabriella Pistilli, Marco Charini, Enzo Tettamanti, Natalia Battista, Mauro Maccarrone, Mauro Mattioli

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: Mammalian spermatozoa acquire their full fertilizing ability (so called capacitation) within the female genital tract, where they are progressively exposed to inverse gradients of inhibiting and stimulating molecules. Methodology/Principal Findings: In the present research, the effect on this process of anandamide, an endocannabinoid that can either activate or inhibit cannabinoid receptors depending on its concentration, and bicarbonate, an oviductal activatory molecule, was assessed, in order to study the role exerted by the type 1 cannabinoid receptor (CB1R) in the process of lipid membrane remodeling crucial to complete capacitation. To this aim, boar sperm were incubated in vitro under capacitating conditions (stimulated by bicarbonate) in the presence or in the absence of methanandamide (Met-AEA), a non-hydrolysable analogue of anandamide. The CB1R involvement was studied by using the specific inhibitor (SR141716) or mimicking its activation by adding a permeable cAMP analogue (8Br-cAMP). By an immunocytochemistry approach it was shown that the Met-AEA inhibits the bicarbonate-dependent translocation of CB1R from the post-equatorial to equatorial region of sperm head. In addition it was found that Met-AEA is able to prevent the bicarbonate-induced increase in membrane disorder and the cholesterol extraction, both preliminary to capacitation, acting through a CB1R-cAMP mediated pathway, as indicated by MC540 and filipin staining, EPR spectroscopy and biochemical analysis on whole membranes (CB1R activity) and on membrane enriched fraction (C/P content and anisotropy). Conclusions/Significance: Altogether, these data demonstrate that the endocannabinoid system strongly inhibits the process of sperm capacitation, acting as membrane stabilizing agent, thus increasing the basic knowledge on capacitation-related signaling and potentially opening new perspectives in diagnostics and therapeutics of male infertility.

Original languageEnglish
Article numbere23038
JournalPLoS One
Volume6
Issue number8
DOIs
Publication statusPublished - 2011

Fingerprint

Cannabinoid Receptors
membrane fluidity
Membrane Fluidity
Fluidity
Bicarbonates
boars
bicarbonates
Spermatozoa
Chemical activation
spermatozoa
Membranes
Endocannabinoids
rimonabant
filipin
Filipin
Sperm Capacitation
Sperm Head
sperm capacitation
Molecules
Excipients

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Barboni, B., Bernabò, N., Palestini, P., Botto, L., Pistilli, M. G., Charini, M., ... Mattioli, M. (2011). Type-1 Cannabinoid receptors reduce membrane fluidity of capacitated boar sperm by impairing their activation by bicarbonate. PLoS One, 6(8), [e23038]. https://doi.org/10.1371/journal.pone.0023038

Type-1 Cannabinoid receptors reduce membrane fluidity of capacitated boar sperm by impairing their activation by bicarbonate. / Barboni, Barbara; Bernabò, Nicola; Palestini, Paola; Botto, Laura; Pistilli, Maria Gabriella; Charini, Marco; Tettamanti, Enzo; Battista, Natalia; Maccarrone, Mauro; Mattioli, Mauro.

In: PLoS One, Vol. 6, No. 8, e23038, 2011.

Research output: Contribution to journalArticle

Barboni, B, Bernabò, N, Palestini, P, Botto, L, Pistilli, MG, Charini, M, Tettamanti, E, Battista, N, Maccarrone, M & Mattioli, M 2011, 'Type-1 Cannabinoid receptors reduce membrane fluidity of capacitated boar sperm by impairing their activation by bicarbonate', PLoS One, vol. 6, no. 8, e23038. https://doi.org/10.1371/journal.pone.0023038
Barboni, Barbara ; Bernabò, Nicola ; Palestini, Paola ; Botto, Laura ; Pistilli, Maria Gabriella ; Charini, Marco ; Tettamanti, Enzo ; Battista, Natalia ; Maccarrone, Mauro ; Mattioli, Mauro. / Type-1 Cannabinoid receptors reduce membrane fluidity of capacitated boar sperm by impairing their activation by bicarbonate. In: PLoS One. 2011 ; Vol. 6, No. 8.
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