Abstract
In vitro T-cell receptor-induced programed cell death in both activated T cells from human immunodeficiency virus-seronegative (HIV-) donors and resting T cells from HIV+ donors was substantially influenced by cytokines. Addition of exogenous recombinant 'type 1' lymphokines interferon γ and interleukin 2 (IL-2), as well as the macrophage-produced IL-12, which favor cell-mediated T-cell responses, blocks both systems of T-lymphocyte programed cell death. In contrast, the 'type 2' lymphokines IL-4 and IL-10, which favor antibody responses, either had no effect or enhanced these systems of in vitro T-cell programed cell death. A role for endogenously produced cytokines was suggested by the inhibition of T-cell receptor-mediated death by antibodies against IL-4 and IL-10 and its enhancement by anti-IL-12 in cultures containing monocytes. These results demonstrate that the functional properties of type 1 and type 2 cytokine classes may be further extended to include their effects on T-cell programed cell death and their possible role in the pathogenesis of HIV infection.
| Original language | English |
|---|---|
| Pages (from-to) | 11811-11815 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 91 |
| Issue number | 25 |
| DOIs | |
| Publication status | Published - Dec 6 1994 |
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ASJC Scopus subject areas
- Genetics
- General
Cite this
Type 1/type 2 cytokine modulation of T-cell programed cell death as a model for human immunodeficiency virus pathogenesis. / Clerici, Mario; Sarin, Apurva; Coffman, Robert L.; Wynn, Thomas A.; Blatt, Stephen P.; Hendrix, Craig W.; Wolf, Stanley F.; Shearer, Gene M.; Henkart, Pierre A.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, No. 25, 06.12.1994, p. 11811-11815.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Type 1/type 2 cytokine modulation of T-cell programed cell death as a model for human immunodeficiency virus pathogenesis
AU - Clerici, Mario
AU - Sarin, Apurva
AU - Coffman, Robert L.
AU - Wynn, Thomas A.
AU - Blatt, Stephen P.
AU - Hendrix, Craig W.
AU - Wolf, Stanley F.
AU - Shearer, Gene M.
AU - Henkart, Pierre A.
PY - 1994/12/6
Y1 - 1994/12/6
N2 - In vitro T-cell receptor-induced programed cell death in both activated T cells from human immunodeficiency virus-seronegative (HIV-) donors and resting T cells from HIV+ donors was substantially influenced by cytokines. Addition of exogenous recombinant 'type 1' lymphokines interferon γ and interleukin 2 (IL-2), as well as the macrophage-produced IL-12, which favor cell-mediated T-cell responses, blocks both systems of T-lymphocyte programed cell death. In contrast, the 'type 2' lymphokines IL-4 and IL-10, which favor antibody responses, either had no effect or enhanced these systems of in vitro T-cell programed cell death. A role for endogenously produced cytokines was suggested by the inhibition of T-cell receptor-mediated death by antibodies against IL-4 and IL-10 and its enhancement by anti-IL-12 in cultures containing monocytes. These results demonstrate that the functional properties of type 1 and type 2 cytokine classes may be further extended to include their effects on T-cell programed cell death and their possible role in the pathogenesis of HIV infection.
AB - In vitro T-cell receptor-induced programed cell death in both activated T cells from human immunodeficiency virus-seronegative (HIV-) donors and resting T cells from HIV+ donors was substantially influenced by cytokines. Addition of exogenous recombinant 'type 1' lymphokines interferon γ and interleukin 2 (IL-2), as well as the macrophage-produced IL-12, which favor cell-mediated T-cell responses, blocks both systems of T-lymphocyte programed cell death. In contrast, the 'type 2' lymphokines IL-4 and IL-10, which favor antibody responses, either had no effect or enhanced these systems of in vitro T-cell programed cell death. A role for endogenously produced cytokines was suggested by the inhibition of T-cell receptor-mediated death by antibodies against IL-4 and IL-10 and its enhancement by anti-IL-12 in cultures containing monocytes. These results demonstrate that the functional properties of type 1 and type 2 cytokine classes may be further extended to include their effects on T-cell programed cell death and their possible role in the pathogenesis of HIV infection.
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U2 - 10.1073/pnas.91.25.11811
DO - 10.1073/pnas.91.25.11811
M3 - Article
VL - 91
SP - 11811
EP - 11815
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 25
ER -