Type 2 diabetes and metabolic syndrome are associated with increased expression of 11β-hydroxysteroid dehydrogenase 1 in obese subjects

L. Alberti, A. Girola, L. Gilardini, A. Conti, S. Cattaldo, G. Micheletto, C. Invitti

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Objective: The role of glucocorticoids production in adipose tissue in the development of metabolic disorders in humans has not been fully characterized. We investigated whether in obese subjects, 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) expression in subcutaneous (SAT) and visceral (VAT) adipose tissue is associated with the occurrence of metabolic disorders and the expression of adiponectin and tumor necrosis factor α (TNFα) and two glucocorticoid-regulated adipokines able to influence the metabolic control. Design and subjects: Sixty-two obese patients were enrolled in the study. SAT and VAT samples were obtained from 13 patients undergoing bariatric surgery (body mass index (BMI) 39.1±5.3 kg/m2). SAT samples were obtained from 49 patients who underwent periumbilical biopsy (BMI 36.9±5.1 kg/m2). Measurements: Oral glucose tolerance tests in subjects without known diabetes. Circulating glucose, lipid, insulin, adiponectin, TNFα and urinary-free cortisol levels. Real-time PCR to quantify mRNA levels of 11β-HSD1, hexose-6-phosphate dehydrogenase (H6PDH), adiponectin and TNFα. Western blot analysis to evaluate 11β-HSD1 protein expression. Results: In the majority of the obese subjects, VAT expresses more 11β-HSD1 than SAT. VAT 11β-HSD1 expression was not associated with metabolic disorders. SAT 11β-HSD1 mRNA levels were higher in subjects with than in those without metabolic syndrome (P

Original languageEnglish
Pages (from-to)1826-1831
Number of pages6
JournalInternational Journal of Obesity
Volume31
Issue number12
DOIs
Publication statusPublished - Dec 2007

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11-beta-Hydroxysteroid Dehydrogenases
hydroxysteroid dehydrogenases
metabolic syndrome
noninsulin-dependent diabetes mellitus
Type 2 Diabetes Mellitus
adiponectin
tumor necrosis factors
Adiponectin
metabolic diseases
Tumor Necrosis Factor-alpha
glucocorticoids
Glucocorticoids
adipose tissue
body mass index
Body Mass Index
bariatric surgery
adipokines
Messenger RNA
Adipokines
Bariatric Surgery

Keywords

  • 11β-HDS 1
  • Adiponectin
  • Adipose tissue
  • TNFα
  • Type 2 diabetes
  • Urinary-free cortisol

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Public Health, Environmental and Occupational Health
  • Endocrinology
  • Food Science
  • Endocrinology, Diabetes and Metabolism

Cite this

Type 2 diabetes and metabolic syndrome are associated with increased expression of 11β-hydroxysteroid dehydrogenase 1 in obese subjects. / Alberti, L.; Girola, A.; Gilardini, L.; Conti, A.; Cattaldo, S.; Micheletto, G.; Invitti, C.

In: International Journal of Obesity, Vol. 31, No. 12, 12.2007, p. 1826-1831.

Research output: Contribution to journalArticle

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