Typing of methicillin-resistant Staphylococcus aureus (MRSA) strains from an intensive care unit by random amplified polymorphic DNA (RAPD)

S. Telecco, D. Barbarini, E. Carretto, S. Comincini, V. Emmi, P. Marone

Research output: Contribution to journalArticlepeer-review

Abstract

The identification and control of methicillin-resistant Staphylococcus aureus (MRSA) is of primary concern in intensive care units (ICUs) worldwide. The introduction and circulation of particular strains is best studied by genomic procedures and random amplified polymorphic DNA (RAPD) is well suited for this task. In this study 14 isolates of MRSA, obtained over an 8 month period from the blood cultures of 12 patients in an ICU at our hospital, were typed by RAPD method using seven primers. Three separate groups were distinguished and clustering of certain types in time and space was noted. These results suggest that although different strains of MRSA were involved in this outbreak, cross-infection with individual types occurred. RAPD fingerprinting is a relatively simple method that allows epidemiologic investigation of MRSA outbreaks in hospital infection.

Original languageEnglish
Pages (from-to)323-329
Number of pages7
JournalNew Microbiologica
Volume22
Issue number4
Publication statusPublished - Oct 1999

Keywords

  • Fingerprinting
  • Infection control surveillance
  • Methicillin-resistant s. Aureus
  • Molecular typing
  • RAPD

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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