Tyrosine kinase receptor indistinguishable from the c-met protein

S. Giordano, C. Ponzetto, M. F. Di Renzo, C. S. Cooper, P. M. Comoglio

Research output: Contribution to journalArticle

Abstract

Growth factor receptors with protein tyrosine kinase activity are central to the control of proliferation of both normal and malignant cells. Using anti-phosphotyrosine antibodies, we have previously identified a transmembrane glycoprotein with abnormally high protein tyrosine kinase activity in a human gastric tumour cell line (GTL-16). Electrophoresis under non-reducing conditions revealed that this kinase (relative molecular mass 145,000 (145 K)) is disulphide-linked to a 50 K chain in an αβ-complex of 190 K (p190). From its novel two-chain structure, we deduced that p190 was the prototype of a new class of tyrosine kinase receptors. We now show that p190 is indistinguishable from the protein encoded by the c-met proto-oncogene and that the αβ-subunit structure is conserved in other human cell lines. We also show that the high level of p190 found in the GTL-16 cell line is accompanied by amplification and overexpression of c-met. This provides the first example of a functional alteration of c-met in a human tumour cell line.

Original languageEnglish
Pages (from-to)155-156
Number of pages2
JournalNature
Volume339
Issue number6220
Publication statusPublished - 1989

ASJC Scopus subject areas

  • General

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    Giordano, S., Ponzetto, C., Di Renzo, M. F., Cooper, C. S., & Comoglio, P. M. (1989). Tyrosine kinase receptor indistinguishable from the c-met protein. Nature, 339(6220), 155-156.