Tyrosine phosphorylation of synapsin I by Src regulates synaptic-vesicle trafficking

Mirko Messa, Sonia Congia, Enrico Defranchi, Flavia Valtorta, Anna Fassio, Franco Onofri, Fabio Benfenati

Research output: Contribution to journalArticle

Abstract

Synapsins are synaptic vesicle (SV)-associated phosphoproteins involved in the regulation of neurotransmitter release. Synapsins reversibly tether SVs to the cytoskeleton and their phosphorylation by serine/threonine kinases increases SV availability for exocytosis by impairing their association with SVs and/or actin. We recently showed that synapsin I, through SH3- or SH2-mediated interactions, activates Src and is phosphorylated by the same kinase at Tyr301. Here, we demonstrate that, in contrast to serine phosphorylation, Src-mediated tyrosine phosphorylation of synapsin I increases its binding to SVs and actin, and increases the formation of synapsin dimers, which are both potentially involved in SV clustering. Synapsin I phosphorylation by Src affected SV dynamics and was physiologically regulated in brain slices in response to depolarization. Expression of the non-phosphorylatable (Y301F) synapsin I mutant in synapsin-I-knockout neurons increased the sizes of the readily releasable and recycling pools of SVs with respect to the wild-type form, which is consistent with an increased availability of recycled SVs for exocytosis. The data provide a mechanism for the effects of Src on SV trafficking and indicate that tyrosine phosphorylation of synapsins, unlike serine phosphorylation, stimulates the reclustering of recycled SVs and their recruitment to the reserve pool.

Original languageEnglish
Pages (from-to)2256-2265
Number of pages10
JournalJournal of Cell Science
Volume123
Issue number13
DOIs
Publication statusPublished - Jul 1 2010

Fingerprint

Synapsins
Synaptic Vesicles
Tyrosine
Phosphorylation
Exocytosis
Serine
Actins
Protein-Serine-Threonine Kinases
Phosphoproteins
Recycling
Cytoskeleton
Neurotransmitter Agents
Cluster Analysis
Phosphotransferases
Neurons

Keywords

  • Actin
  • Exo-endocytosis
  • Neurotransmitter release
  • Neurotrophins
  • Synaptic plasticity
  • Tyrosine kinases

ASJC Scopus subject areas

  • Cell Biology
  • Medicine(all)

Cite this

Messa, M., Congia, S., Defranchi, E., Valtorta, F., Fassio, A., Onofri, F., & Benfenati, F. (2010). Tyrosine phosphorylation of synapsin I by Src regulates synaptic-vesicle trafficking. Journal of Cell Science, 123(13), 2256-2265. https://doi.org/10.1242/jcs.068445

Tyrosine phosphorylation of synapsin I by Src regulates synaptic-vesicle trafficking. / Messa, Mirko; Congia, Sonia; Defranchi, Enrico; Valtorta, Flavia; Fassio, Anna; Onofri, Franco; Benfenati, Fabio.

In: Journal of Cell Science, Vol. 123, No. 13, 01.07.2010, p. 2256-2265.

Research output: Contribution to journalArticle

Messa, M, Congia, S, Defranchi, E, Valtorta, F, Fassio, A, Onofri, F & Benfenati, F 2010, 'Tyrosine phosphorylation of synapsin I by Src regulates synaptic-vesicle trafficking', Journal of Cell Science, vol. 123, no. 13, pp. 2256-2265. https://doi.org/10.1242/jcs.068445
Messa, Mirko ; Congia, Sonia ; Defranchi, Enrico ; Valtorta, Flavia ; Fassio, Anna ; Onofri, Franco ; Benfenati, Fabio. / Tyrosine phosphorylation of synapsin I by Src regulates synaptic-vesicle trafficking. In: Journal of Cell Science. 2010 ; Vol. 123, No. 13. pp. 2256-2265.
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