UbcH10 expression on thyroid fine-needle aspirates

Eliana Guerriero, Angelo Ferraro, Doriana Desiderio, Pierlorenzo Pallante, Maria Teresa Berlingieri, Antonino Iaccarino, Emiliano Palmieri, Lucio Palombini, Alfredo Fusco, Giancarlo Troncone

Research output: Contribution to journalArticlepeer-review


BACKGROUND: Thyroid fine-needle aspiration (FNA) samples belonging to the follicular neoplasm/suspicious for malignancy classes are controversial. The authors identified UbcH10 as a marker useful in the diagnosis of several neoplasms, including thyroid cancer. Here, analysis of UbcH10 expression by quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemistry was applied to FNAs. METHODS: A series of 84 follicular neoplasm/suspicious for malignancy FNAs with histological follow- up (30 malignant) was prospectively collected. UbcH10 immunostaining was performed on cell blocks and compared with that of the proliferation marker Ki-67. At the mRNA level, UbcH10 was compared with CCND2 and PCSK2 expression, these latter being the best performing components of the previously reported 3-gene assay; to determine the diagnostic accuracy, the area under the curve (AUC) of the receiver operating characteristic (ROC) curve for each gene individually and in combination was evaluated. RESULTS: UbcH10 and Ki-67 shared a similar pattern; although UbcH10 expression was higher in malignant than in benign lesions (P <.001), staining was sporadic, and the cutoff value derived by the ROC analysis was too low (1.25%) for routine application. Conversely, UbcH10 expression assessment by quantitative RTPCR was effective. UbcH10 mRNA levels associated with malignant histology were significantly higher than those associated with benign histology (P = .02). The AUC was 0.74 for UbcH10, 0.81 for CCDN2, 0.62 for PCSK2, and 0.84 for UbcH10 and CCND2 combination. CONCLUSIONS: UbcH10 quantitative RT-PCR analysis, rather than immunohistochemistry, is useful to increase the detection of malignancy in thyroid FNAs. UbcH10 may be added as a panel component in quantitative RT-PCR-based assays.

Original languageEnglish
Pages (from-to)157-165
Number of pages9
JournalCancer cytopathology
Issue number3
Publication statusPublished - 2010


  • 3-Gene assay
  • Fine-needle aspiration
  • Ki-67
  • Thyroid
  • UbcH10

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Medicine(all)


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