UCP4C mediates uncoupled respiration in larvae of Drosophila melanogaster

Caterina Da-Ré, Cristiano De Pittà, Mauro A. Zordan, Giordano Teza, Fabrizio Nestola, Massimo Zeviani, Rodolfo Costa, Paolo Bernardi

Research output: Contribution to journalArticlepeer-review

Abstract

Larvae of Drosophila melanogaster reared at 23°C and switched to 14°C for 1 h are 0.5°C warmer than the surrounding medium. In keeping with dissipation of energy, respiration of Drosophila melanogaster larvae cannot be decreased by the F-ATPase inhibitor oligomycin or stimulated by protonophore. Silencing of Ucp4C conferred sensitivity of respiration to oligomycin and uncoupler, and prevented larva-to-adult progression at 15°C but not 23°C. Uncoupled respiration of larval mitochondria required palmitate, was dependent on Ucp4C and was inhibited by guanosine diphosphate. UCP4C is required for development through the prepupal stages at low temperatures and may be an uncoupling protein. Synopsis Larvae of Drosophila melanogaster kept in the cold are warmer than the surrounding medium. In keeping with dissipation of energy, respiration of larvae is uncoupled. Knockdown of UCP4C, a homolog of the mammalian uncoupling protein UCP1, confers larvae with coupled respiration. UCP4C is required for development through the prepupal stages at low temperature. Larvae of Drosophila melanogaster kept in the cold are warmer than the surrounding medium. In keeping with dissipation of energy, respiration of larvae is uncoupled.

Original languageEnglish
Pages (from-to)586-591
Number of pages6
JournalEMBO Reports
Volume15
Issue number5
DOIs
Publication statusPublished - 2014

Keywords

  • Drosophila melanogaster
  • energy dissipation
  • mitochondria
  • respiration
  • uncoupling proteins

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biochemistry

Fingerprint Dive into the research topics of 'UCP4C mediates uncoupled respiration in larvae of Drosophila melanogaster'. Together they form a unique fingerprint.

Cite this