UDP exerts cytostatic and cytotoxic actions in human neuroblastoma SH-SY5Y cells over-expressing P2Y6 receptor

Savina Apolloni, Pamela Finocchi, Igea D'Agnano, Susanna Alloisio, Mario Nobile, Nadia D'Ambrosi, Cinzia Volonté

Research output: Contribution to journalArticlepeer-review

Abstract

The role of P2 receptors for purines/pyrimidines is not well characterized in neuroblastoma, although a variety of purinergic mRNAs/proteins are expressed in these cells. Among these, the P2Y6 receptor is the only subtype distinguished by UDP-specific activation. In this work, after over-expressing the P2Y6 protein in human neuroblastoma SH-SY5Y cells, we find that UDP arrests cell cycle and induces apoptosis, by counteracting the pathological functioning of neuroblastoma in vitro. UDP also causes mitochondrial damage through diffusion of cytochrome c in the cytoplasm, and stimulates caspase-3,7,8 activities, with extensive over-expression of manganese superoxide dismutase. Our data establish the direct toxic role and anti-cancer activity of UDP in a neuroblastoma cell line, and identify the P2Y6 receptor as a novel potential target in anti-tumoural therapies. This constitutes an advancement not only in the knowledge of purinergic signalling, but also in the biological and pathological aspects of neuroblastoma in vitro.

Original languageEnglish
Pages (from-to)670-678
Number of pages9
JournalNeurochemistry International
Volume56
Issue number5
DOIs
Publication statusPublished - Apr 2010

Keywords

  • Apoptosis
  • Cancer
  • Caspase
  • Extracellular ATP
  • Purinergic receptor

ASJC Scopus subject areas

  • Cell Biology
  • Cellular and Molecular Neuroscience

Fingerprint

Dive into the research topics of 'UDP exerts cytostatic and cytotoxic actions in human neuroblastoma SH-SY5Y cells over-expressing P2Y6 receptor'. Together they form a unique fingerprint.

Cite this