Ultrastructural changes in rat cornea after prolonged hypobaric hypoxia

L. Mastropasqua, M. Ciancaglini, G. Di Tano, P. Carpineto, L. Lobefalo, B. Loffredo, D. Bosco, M. Columbaro, E. Falcieri

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A study was performed to evaluate the effect of prolonged environmental hypobaric hypoxia on the ultrastructure of rat cornea. 60-day-old female Wistar rats were exposed to a simulated altitude of 5,500 m (350 mmHg) and pO2 of 76 mmHg for 30 days. Control rats were exposed to atmospheric sea level pressure (760 mmHg) and pO2 of 150 mmHg, for the same period. Ultrastructural analysis of the cornea epithelium did not reveal any relevant difference between control and treated rats. In contrast, the corneal stroma of rats subjected to hypoxia showed vascularization with advanced vessel differentiation and signs of active proliferation. The endothelium of hypoxic cornea showed swollen mitochondria and large empty cytoplasmic areas. The endothelial intercellular junctions could hardly be identified in the hypoxic condition. Nevertheless, the most evident change in hypoxic cornea was in Descemet's membrane, which was considerably thickened, to approximately twice that of the control specimen. These results suggest that environmental hypobaric hypoxia induces marked alterations in the corneal stroma and endothelium morphology, probably related to reduced oxygen tension in the aqueous humor, consequent to hypoxemia.

Original languageEnglish
Pages (from-to)285-293
Number of pages9
JournalJournal of Submicroscopic Cytology and Pathology
Volume30
Issue number2
Publication statusPublished - Apr 1998

Fingerprint

Cornea
Corneal Stroma
Descemet Membrane
Corneal Endothelium
Intercellular Junctions
Aqueous Humor
Oceans and Seas
Endothelium
Wistar Rats
Mitochondria
Epithelium
Oxygen
Pressure
Hypoxia

Keywords

  • Hypobaric hypoxia
  • Rat cornea
  • Ultrastructure

ASJC Scopus subject areas

  • Cell Biology
  • Histology
  • Pathology and Forensic Medicine

Cite this

Mastropasqua, L., Ciancaglini, M., Di Tano, G., Carpineto, P., Lobefalo, L., Loffredo, B., ... Falcieri, E. (1998). Ultrastructural changes in rat cornea after prolonged hypobaric hypoxia. Journal of Submicroscopic Cytology and Pathology, 30(2), 285-293.

Ultrastructural changes in rat cornea after prolonged hypobaric hypoxia. / Mastropasqua, L.; Ciancaglini, M.; Di Tano, G.; Carpineto, P.; Lobefalo, L.; Loffredo, B.; Bosco, D.; Columbaro, M.; Falcieri, E.

In: Journal of Submicroscopic Cytology and Pathology, Vol. 30, No. 2, 04.1998, p. 285-293.

Research output: Contribution to journalArticle

Mastropasqua, L, Ciancaglini, M, Di Tano, G, Carpineto, P, Lobefalo, L, Loffredo, B, Bosco, D, Columbaro, M & Falcieri, E 1998, 'Ultrastructural changes in rat cornea after prolonged hypobaric hypoxia', Journal of Submicroscopic Cytology and Pathology, vol. 30, no. 2, pp. 285-293.
Mastropasqua L, Ciancaglini M, Di Tano G, Carpineto P, Lobefalo L, Loffredo B et al. Ultrastructural changes in rat cornea after prolonged hypobaric hypoxia. Journal of Submicroscopic Cytology and Pathology. 1998 Apr;30(2):285-293.
Mastropasqua, L. ; Ciancaglini, M. ; Di Tano, G. ; Carpineto, P. ; Lobefalo, L. ; Loffredo, B. ; Bosco, D. ; Columbaro, M. ; Falcieri, E. / Ultrastructural changes in rat cornea after prolonged hypobaric hypoxia. In: Journal of Submicroscopic Cytology and Pathology. 1998 ; Vol. 30, No. 2. pp. 285-293.
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