Ultrastructural features and P-glycoprotein immunolocalization in Saos-2/DX580 multidrug-resistant human osteosarcoma cells

N. M. Maraldi, N. Zini, P. Sabatelli, A. Valmori, K. Scotlandi, M. Serra, N. Baldini

Research output: Contribution to journalArticlepeer-review

Abstract

The multiple drug type of resistance to anticancer agents (MDR) is mediated by an overexpression of the MDR1 gene product, the P-glycoprotein. This is largely present at the cell surface of MDR cells, mediating the active efflux of cytotoxic molecules, but may be found also intracellularly. In this paper, using Saos-2 human osteosarcoma cells as a model, we provide further evidence of increased presence of P-glycoprotein at the plasma membrane and in the nucleus of MDR cells, where it is closely bound to the nuclear matrix. The structural changes observed in Saos-2 MDR cells, including an increase of the cell surface by the formation of blebs, and a peculiar clustering of chromatin, which are similar to those observed in other MDR cell lines, are likely to be associated with the observed overexpression of the P-glycoprotein at the cell membrane and nuclear level. These findings suggest the existence of more complex, still undetermined, mechanisms underlying the MDR phenomenon.

Original languageEnglish
Pages (from-to)93-100
Number of pages8
JournalJournal of Submicroscopic Cytology and Pathology
Volume28
Issue number1
Publication statusPublished - 1996

Keywords

  • Electron microscopy
  • Human osteosarcoma
  • Immunogold
  • Multidrug resistance
  • Nuclear matrix
  • P-glycoprotein

ASJC Scopus subject areas

  • Cell Biology
  • Histology
  • Pathology and Forensic Medicine

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