Ultrastructural features of the isolated guinea-pig brain maintained in vitro by arterial perfusion

M. de Curtis, P. Arcelli, S. de Biasi, R. Spreafico, G. Avanzini

Research output: Contribution to journalArticle

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Abstract

The morphological features of cerebral tissue in the isolated guinea-pig brain maintained in vitro by arterial perfusion are described. Light and electron microscopic anatysis of the thalamus, the somatosensory cortex and the limbic cortices (hippocampus, piriform and entorhinal cortices) was performed after different periods of incubation in vitro (1,7 and 12 h), in parallel with an electrophysiological study. The morphological analysis showed that neuronal elements retained their normal appearance at both cellular and subcellular level in the examined brain regions up to an incubation period of 12 h. Immunoreactivity for GABA was also preserved for up to 12 h of in vitro perfusion. Vasogenic edema and perivascular extracellular swelling appeared after 7 h, together with signs of progressive astrocytic deterioration. These findings show that normal electrophysiological recordings correlate with good anatomical preservation of the isolated guinea-pig brain preparation after prolonged times of arterial in vitro perfusion.

Original languageEnglish
Pages (from-to)775-788
Number of pages14
JournalNeuroscience
Volume59
Issue number3
DOIs
Publication statusPublished - 1994

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Guinea Pigs
Perfusion
Brain
Entorhinal Cortex
Somatosensory Cortex
Thalamus
gamma-Aminobutyric Acid
Hippocampus
Edema
Electrons
Light
In Vitro Techniques

ASJC Scopus subject areas

  • Neuroscience(all)

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Ultrastructural features of the isolated guinea-pig brain maintained in vitro by arterial perfusion. / de Curtis, M.; Arcelli, P.; de Biasi, S.; Spreafico, R.; Avanzini, G.

In: Neuroscience, Vol. 59, No. 3, 1994, p. 775-788.

Research output: Contribution to journalArticle

de Curtis, M. ; Arcelli, P. ; de Biasi, S. ; Spreafico, R. ; Avanzini, G. / Ultrastructural features of the isolated guinea-pig brain maintained in vitro by arterial perfusion. In: Neuroscience. 1994 ; Vol. 59, No. 3. pp. 775-788.
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