Ultrastructural identification of sulphated glycoconjugates in the Golgi apparatus in human colonic absorptive cells

F. Malchiodi Albedi, A. M. Cassano, F. Ciaralli, D. Taruscio, G. Donelli

Research output: Contribution to journalArticle

Abstract

The subcellular localization of sulphated glycoconjugates was determined at the ultrastructural level by using the high iron diamine (HID) technique for sulphate groups in the absorptive cells of human colonic mucosa. Stained material was observed on the apical plasma membrane, in intracytoplasmic vesicles and in the Golgi complex. In this organelle, the last two or three cisternae of the trans side and the trans-Golgi network (TGN) were labelled, as well as a variable number of coated and noncoated vesicles facing the trans side and surrounding trans-Golgi network. These findings point to the trans side of the Golgi apparatus and trans-Golgi network as the subcompartments functionally involved in the sulphation of glycoconjugates.

Original languageEnglish
Pages (from-to)73-79
Number of pages7
JournalHistochemistry
Volume92
Issue number1
DOIs
Publication statusPublished - Jan 1989

    Fingerprint

ASJC Scopus subject areas

  • Anatomy

Cite this