Ultrastructural immunolocalization of GAP-43 in the sprouted mossy fibres of kainic acid lesioned rats

Caterina Bendotti, Sonia Baldessari, Rosario Samanin, Silvia De Biasi

Research output: Contribution to journalArticlepeer-review

Abstract

Kainic acid (KA)-induced seizures in adult rats have been reported to cause sprouting of mossy fibres in the inner molecular layer (IML) of the dentate gyrus. In the present study, using electron microscopic immunostaining for GAP-43 we found that 3 months after KA treatment, several GAP-43 positive terminals in the IML showed structural characteristics of mossy fibre boutons. No GAP-43-positive mossy fibre terminals were found in the normal projection areas of granule cells, thus indicating that newly synthesized GAP-43 is transported predominantly in the axonal branches actively undergoing remodelling. These results provide evidence of the involvement of GAP-43 in the structural remodelling of mossy fibres, and suggest a role of this protein in the functional activity of the sprouted mossy fibres as a consequence of KA-induced seizures.

Original languageEnglish
Pages (from-to)2645-2648
Number of pages4
JournalNeuroReport
Volume5
Issue number18
Publication statusPublished - 1994

Keywords

  • Dentate gyrus
  • Epilepsy
  • GAP-43
  • Hippocampus
  • Immunocytochemistry
  • Kainic acid
  • Neuronal plasticity
  • Rats
  • Sprouting

ASJC Scopus subject areas

  • Neuroscience(all)

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