Ultrastructural localization of calcium binding sites on human muscle cell surface

M. Moggio, S. Jann, L. Adobbati, A. Prelle, A. Gallanti, G. Fagiolari, G. Pellegrini, G. Scarlato

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Calcium (Ca2+) is mainly bound to anionic phospholipids and to sialic acid at the cell surface. We studied the ultrastructural localization of these Ca2+ binding sites in normal human muscle fibers, using Polymyxin B as a marker for anionic phospholipids and the lectin Limulus Polyphemus as a probe for sialic acid. We found that anionic phospholipids have a patchy distribution along the muscle sarcolemma, with a preferential localization at the I band level and at the junction between the I and A band. Sialic acid has an uniform distribution along the muscle plasma membrane and basal lamina. Our observations suggest that the plasma membrane, basal lamina, and transverse tubular system play an important role in providing the negative charge of the human muscle cell surface and that these structures may be involved in the binding of calcium.

Original languageEnglish
Pages (from-to)910-914
Number of pages5
JournalMuscle and Nerve
Volume12
Issue number11
Publication statusPublished - 1989

Fingerprint

N-Acetylneuraminic Acid
Muscle Cells
Phospholipids
Binding Sites
Calcium
Basement Membrane
Muscles
Cell Membrane
Horseshoe Crabs
Polymyxin B
Sarcolemma
Lectins

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Moggio, M., Jann, S., Adobbati, L., Prelle, A., Gallanti, A., Fagiolari, G., ... Scarlato, G. (1989). Ultrastructural localization of calcium binding sites on human muscle cell surface. Muscle and Nerve, 12(11), 910-914.

Ultrastructural localization of calcium binding sites on human muscle cell surface. / Moggio, M.; Jann, S.; Adobbati, L.; Prelle, A.; Gallanti, A.; Fagiolari, G.; Pellegrini, G.; Scarlato, G.

In: Muscle and Nerve, Vol. 12, No. 11, 1989, p. 910-914.

Research output: Contribution to journalArticle

Moggio, M, Jann, S, Adobbati, L, Prelle, A, Gallanti, A, Fagiolari, G, Pellegrini, G & Scarlato, G 1989, 'Ultrastructural localization of calcium binding sites on human muscle cell surface', Muscle and Nerve, vol. 12, no. 11, pp. 910-914.
Moggio M, Jann S, Adobbati L, Prelle A, Gallanti A, Fagiolari G et al. Ultrastructural localization of calcium binding sites on human muscle cell surface. Muscle and Nerve. 1989;12(11):910-914.
Moggio, M. ; Jann, S. ; Adobbati, L. ; Prelle, A. ; Gallanti, A. ; Fagiolari, G. ; Pellegrini, G. ; Scarlato, G. / Ultrastructural localization of calcium binding sites on human muscle cell surface. In: Muscle and Nerve. 1989 ; Vol. 12, No. 11. pp. 910-914.
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