Ultrastructural localization of extracellular immunoglobulins in immune-complex-mediated glomerulonephritis - Immunoelectron microscopy of Epon-embedded human renal biopsies using the immunogold staining procedure

G. Viale, P. Dell'Orto, R. Colombi, G. Coggi

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Abstract

The ultrastructural localization of immunoglobulins (Igs) in immunologically mediated glomerulonephritis was achieved in ultrathin sections of glutaraldehyde-fixed, post-osmicated, Epon-embedded human renal needle biopsies using the indirect immunogold staining procedure. Extracellular Igs were identified in electron-dense deposits in subendothelia, intramembranous, subepithelial and mesangial locations in different cases of immune-complex-mediated glomerulonephritis. The excellent preservation of the ultrastructural morphology of the tissue sections made it possible to precisely correlate the presence and distribution of the antigens with those of the electron-dense deposits, which are the ultrastructural hallmarks of these diseases. Our post-embedding staining procedure which allows extracellular Igs to be accurately localized should be a very useful tool for immunomorphological investigations of glomerulo-nephritis.

Original languageEnglish
Pages (from-to)1-4
Number of pages4
JournalHistochemistry
Volume84
Issue number1
DOIs
Publication statusPublished - Jan 1986

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Immunoelectron Microscopy
Glomerulonephritis
Antigen-Antibody Complex
Immunoglobulins
Staining and Labeling
Kidney
Biopsy
Electrons
Nephritis
Glutaral
Needle Biopsy
Antigens
EPON

ASJC Scopus subject areas

  • Anatomy

Cite this

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title = "Ultrastructural localization of extracellular immunoglobulins in immune-complex-mediated glomerulonephritis - Immunoelectron microscopy of Epon-embedded human renal biopsies using the immunogold staining procedure",
abstract = "The ultrastructural localization of immunoglobulins (Igs) in immunologically mediated glomerulonephritis was achieved in ultrathin sections of glutaraldehyde-fixed, post-osmicated, Epon-embedded human renal needle biopsies using the indirect immunogold staining procedure. Extracellular Igs were identified in electron-dense deposits in subendothelia, intramembranous, subepithelial and mesangial locations in different cases of immune-complex-mediated glomerulonephritis. The excellent preservation of the ultrastructural morphology of the tissue sections made it possible to precisely correlate the presence and distribution of the antigens with those of the electron-dense deposits, which are the ultrastructural hallmarks of these diseases. Our post-embedding staining procedure which allows extracellular Igs to be accurately localized should be a very useful tool for immunomorphological investigations of glomerulo-nephritis.",
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T1 - Ultrastructural localization of extracellular immunoglobulins in immune-complex-mediated glomerulonephritis - Immunoelectron microscopy of Epon-embedded human renal biopsies using the immunogold staining procedure

AU - Viale, G.

AU - Dell'Orto, P.

AU - Colombi, R.

AU - Coggi, G.

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N2 - The ultrastructural localization of immunoglobulins (Igs) in immunologically mediated glomerulonephritis was achieved in ultrathin sections of glutaraldehyde-fixed, post-osmicated, Epon-embedded human renal needle biopsies using the indirect immunogold staining procedure. Extracellular Igs were identified in electron-dense deposits in subendothelia, intramembranous, subepithelial and mesangial locations in different cases of immune-complex-mediated glomerulonephritis. The excellent preservation of the ultrastructural morphology of the tissue sections made it possible to precisely correlate the presence and distribution of the antigens with those of the electron-dense deposits, which are the ultrastructural hallmarks of these diseases. Our post-embedding staining procedure which allows extracellular Igs to be accurately localized should be a very useful tool for immunomorphological investigations of glomerulo-nephritis.

AB - The ultrastructural localization of immunoglobulins (Igs) in immunologically mediated glomerulonephritis was achieved in ultrathin sections of glutaraldehyde-fixed, post-osmicated, Epon-embedded human renal needle biopsies using the indirect immunogold staining procedure. Extracellular Igs were identified in electron-dense deposits in subendothelia, intramembranous, subepithelial and mesangial locations in different cases of immune-complex-mediated glomerulonephritis. The excellent preservation of the ultrastructural morphology of the tissue sections made it possible to precisely correlate the presence and distribution of the antigens with those of the electron-dense deposits, which are the ultrastructural hallmarks of these diseases. Our post-embedding staining procedure which allows extracellular Igs to be accurately localized should be a very useful tool for immunomorphological investigations of glomerulo-nephritis.

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