Abstract
Despite the large bulk of literature on electroporation, there is a lack of knowledge about the cellular mechanism(s) and subcellular components controlling this phenomenon. In this chapter, we describe a number of ultrastructural alterations occurring in the tissues and in the cellular membranes after exposure to high voltage pulses. Specifically, experimental and ultrastructural data coming from literature body on transdermal drug delivery are briefly discussed. Moreover, alterations following the exposure of orthotopic melanomas and red blood cells to trains of biphasic pulses are reported. To look insight the intimate mechanism(s) controlling electroporation, several imaging techniques such as light and electron microscopy, electrochemical imaging, confocal microscopy have been employed. Freeze-fracture analysis allowed in more than one case to evidence alterations of cell membranes and defects in the dynamic assembly of lipids and proteins. Such modifications could be the hallmarks of a reduction of lipid-protein cohesion and of changes in lipid orientation inside cell membranes, as postulated in several mathematical models applied to electroporation.
| Original language | English |
|---|---|
| Title of host publication | Electroporation in Laboratory and Clinical Investigations |
| Publisher | Nova Science Publishers, Inc. |
| Pages | 115-131 |
| Number of pages | 17 |
| ISBN (Print) | 9781616683276 |
| Publication status | Published - 2011 |
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Keywords
- Electrochemotherapy
- Erythrocytes
- Freeze-fracturing
- Light and electron microscopy
- Nude mouse
- Stratum corneum
- Thin sectioning
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Ultrastructural modifications induced by "electroporation". / Molinari, Agnese; Arancia, Giuseppe; Spugnini, Enrico P.
Electroporation in Laboratory and Clinical Investigations. Nova Science Publishers, Inc., 2011. p. 115-131.Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Ultrastructural modifications induced by "electroporation"
AU - Molinari, Agnese
AU - Arancia, Giuseppe
AU - Spugnini, Enrico P.
PY - 2011
Y1 - 2011
N2 - Despite the large bulk of literature on electroporation, there is a lack of knowledge about the cellular mechanism(s) and subcellular components controlling this phenomenon. In this chapter, we describe a number of ultrastructural alterations occurring in the tissues and in the cellular membranes after exposure to high voltage pulses. Specifically, experimental and ultrastructural data coming from literature body on transdermal drug delivery are briefly discussed. Moreover, alterations following the exposure of orthotopic melanomas and red blood cells to trains of biphasic pulses are reported. To look insight the intimate mechanism(s) controlling electroporation, several imaging techniques such as light and electron microscopy, electrochemical imaging, confocal microscopy have been employed. Freeze-fracture analysis allowed in more than one case to evidence alterations of cell membranes and defects in the dynamic assembly of lipids and proteins. Such modifications could be the hallmarks of a reduction of lipid-protein cohesion and of changes in lipid orientation inside cell membranes, as postulated in several mathematical models applied to electroporation.
AB - Despite the large bulk of literature on electroporation, there is a lack of knowledge about the cellular mechanism(s) and subcellular components controlling this phenomenon. In this chapter, we describe a number of ultrastructural alterations occurring in the tissues and in the cellular membranes after exposure to high voltage pulses. Specifically, experimental and ultrastructural data coming from literature body on transdermal drug delivery are briefly discussed. Moreover, alterations following the exposure of orthotopic melanomas and red blood cells to trains of biphasic pulses are reported. To look insight the intimate mechanism(s) controlling electroporation, several imaging techniques such as light and electron microscopy, electrochemical imaging, confocal microscopy have been employed. Freeze-fracture analysis allowed in more than one case to evidence alterations of cell membranes and defects in the dynamic assembly of lipids and proteins. Such modifications could be the hallmarks of a reduction of lipid-protein cohesion and of changes in lipid orientation inside cell membranes, as postulated in several mathematical models applied to electroporation.
KW - Electrochemotherapy
KW - Erythrocytes
KW - Freeze-fracturing
KW - Light and electron microscopy
KW - Nude mouse
KW - Stratum corneum
KW - Thin sectioning
UR - http://www.scopus.com/inward/record.url?scp=84891997817&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84891997817&partnerID=8YFLogxK
M3 - Chapter
AN - SCOPUS:84891997817
SN - 9781616683276
SP - 115
EP - 131
BT - Electroporation in Laboratory and Clinical Investigations
PB - Nova Science Publishers, Inc.
ER -