Ultrastructural organization of freeze-fractured interphase nuclei.

N. M. Maraldi, F. Marinelli, A. Galanzi, N. Zini, A. Ognibene, R. Del Coco, A. Matteucci, S. Papa

Research output: Contribution to journalArticle

Abstract

The morphology of intact or membrane-deprived interphase nuclei has been analysed by freeze-fracture electron microscopy. This method appears particularly useful for providing information on the distribution and organisation of chromatin and ribonucleoproteins in the absence of dehydration and embedding artifacts of conventional electron microscope techniques which, among other effects, appear to affect heterochromatin distribution, inducing its aggregation along the nuclear envelope. The main levels of chromatin superstructure, from nucleosome to solenoid fibres, are detectable in the replicas of freeze-fractured nuclei on the basis of the size of their shadow, a parameter particularly suitable for automated image analyses.

Original languageEnglish
Pages (from-to)31-39
Number of pages9
JournalBiology of the cell / under the auspices of the European Cell Biology Organization
Volume56
Issue number1
Publication statusPublished - 1986

ASJC Scopus subject areas

  • Cell Biology

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  • Cite this

    Maraldi, N. M., Marinelli, F., Galanzi, A., Zini, N., Ognibene, A., Del Coco, R., Matteucci, A., & Papa, S. (1986). Ultrastructural organization of freeze-fractured interphase nuclei. Biology of the cell / under the auspices of the European Cell Biology Organization, 56(1), 31-39.