Ultrastructural patterns of the α-naphthyl butyrate esterase activity in normal and leukaemic peripheral blood leucocytes

C. Fenoglio, R. Invernizzi, G. Gerzeli

Research output: Contribution to journalArticle

Abstract

The activity of α-naphthyl butyrate esterase was investigated at the ultrastructural level in normal human peripheral blood and in a few cases of hairy cell leukaemia, B-chronic lymphocytic leukaemia and acute monocytic leukaemia. A membrane reactivity was detected in most normal monocytes and lymphocytes. The activity in monocytes was very strong and was inhibited by NaF. It was NaF-resistant and less intense in lymphocytes. The reaction product was localized in the cytoplasm only in a small percentage of lymphocytes. In lymphocytes and monoblasts from pathological samples the pattern of reactivity was similar to that found in their normal counterparts, except for a lower intensity. The hairy cells showed a discrete distribution of the NaF-resistant reaction product on their cell surface. The different patterns of enzyme distribution are discussed critically.

Original languageEnglish
Pages (from-to)595-604
Number of pages10
JournalHistochemical Journal
Volume17
Issue number5
DOIs
Publication statusPublished - May 1985

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Fingerprint Dive into the research topics of 'Ultrastructural patterns of the α-naphthyl butyrate esterase activity in normal and leukaemic peripheral blood leucocytes'. Together they form a unique fingerprint.

  • Cite this