The peripheral leukocytes of 6 patients with acute leukemia were studied using ultrastructural cytochemical methods which demonstrate myeloperoxidase activity. In some cases there was a discrepancy between submicroscopic positivity and negativity at the light microscope; this could however be due to the small size and low number of cytoplasmic granulation of the leukemic cells. On the other hand it is not always possible to make an exact cytological classification of acute leukemias using ultrastructural cytochemistry; the negativity of the peroxidase reaction could in fact depend not only on the cell line but also on a deficit in production or activity of the enzyme in the leukemic cell. Lastly, when evaluating the results it is necessary to bear in mind that artifacts may be produced when submicroscopical methods are used to demonstrate particular enzymatic activities.
|Title of host publication||Journal of Submicroscopic Cytology|
|Number of pages||9|
|Publication status||Published - 1978|
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