Ultrastructure of neurovascular changes in human diabetic retinopathy

János Fehér, Samanta Taurone, Marialuisa Spoletini, Zsolt Biró, Balázs Varsányi, Gianluca Scuderi, Maria Patrizia Orlando, Rosaria Turchetta, Alessandra Micera, Marco Artico

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The previous concept regarding diabetic retinopathy assigned a primary role to hyperglycemia-induced microvascular alterations, while neuronal and glial abnormalities were considered to be secondary to either ischemia or exudation. The aim of this study was to reveal the potential role of neuronal and glial cells in initial and advanced alterations of the retinopathy in human type 2 diabetes. Electron microscopy and histochemical studies were performed on 38 surgically removed human eyes (28 obtained from diabetic patients and 10 from non-diabetic patients). Morphometric analysis of basement membrane material and lipids was performed. An accumulation of metabolic by-products was found in the capillary wall with aging: this aspect was significantly more pronounced in diabetics. Müller glial cells were found to contribute to alterations of the capillary wall and to occlusion, as well as to the development of proliferative retinopathy and cystoid degeneration of the retina. Our results showed morphological evidence regarding the role of neuronal and glial cells in the pathology of diabetic retinopathy, prior and in addition to microangiopathy. These morphological findings support a neurovascular pathogenesis at the origin of diabetic retinopathy, thus the current treatment approach should be completed by neuroprotective measures.

Original languageEnglish
Pages (from-to)394632017748841
JournalInternational Journal of Immunopathology and Pharmacology
DOIs
Publication statusE-pub ahead of print - Dec 1 2017

Fingerprint

Diabetic Retinopathy
Neuroglia
Membrane Lipids
Basement Membrane
Hyperglycemia
Type 2 Diabetes Mellitus
Retina
Electron Microscopy
Ischemia
Pathology
Therapeutics

Keywords

  • Journal Article

Cite this

Fehér, J., Taurone, S., Spoletini, M., Biró, Z., Varsányi, B., Scuderi, G., ... Artico, M. (2017). Ultrastructure of neurovascular changes in human diabetic retinopathy. International Journal of Immunopathology and Pharmacology, 394632017748841. https://doi.org/10.1177/0394632017748841

Ultrastructure of neurovascular changes in human diabetic retinopathy. / Fehér, János; Taurone, Samanta; Spoletini, Marialuisa; Biró, Zsolt; Varsányi, Balázs; Scuderi, Gianluca; Orlando, Maria Patrizia; Turchetta, Rosaria; Micera, Alessandra; Artico, Marco.

In: International Journal of Immunopathology and Pharmacology, 01.12.2017, p. 394632017748841.

Research output: Contribution to journalArticle

Fehér, J, Taurone, S, Spoletini, M, Biró, Z, Varsányi, B, Scuderi, G, Orlando, MP, Turchetta, R, Micera, A & Artico, M 2017, 'Ultrastructure of neurovascular changes in human diabetic retinopathy', International Journal of Immunopathology and Pharmacology, pp. 394632017748841. https://doi.org/10.1177/0394632017748841
Fehér, János ; Taurone, Samanta ; Spoletini, Marialuisa ; Biró, Zsolt ; Varsányi, Balázs ; Scuderi, Gianluca ; Orlando, Maria Patrizia ; Turchetta, Rosaria ; Micera, Alessandra ; Artico, Marco. / Ultrastructure of neurovascular changes in human diabetic retinopathy. In: International Journal of Immunopathology and Pharmacology. 2017 ; pp. 394632017748841.
@article{6ae8f9c1958347debdea648cfecc6741,
title = "Ultrastructure of neurovascular changes in human diabetic retinopathy",
abstract = "The previous concept regarding diabetic retinopathy assigned a primary role to hyperglycemia-induced microvascular alterations, while neuronal and glial abnormalities were considered to be secondary to either ischemia or exudation. The aim of this study was to reveal the potential role of neuronal and glial cells in initial and advanced alterations of the retinopathy in human type 2 diabetes. Electron microscopy and histochemical studies were performed on 38 surgically removed human eyes (28 obtained from diabetic patients and 10 from non-diabetic patients). Morphometric analysis of basement membrane material and lipids was performed. An accumulation of metabolic by-products was found in the capillary wall with aging: this aspect was significantly more pronounced in diabetics. M{\"u}ller glial cells were found to contribute to alterations of the capillary wall and to occlusion, as well as to the development of proliferative retinopathy and cystoid degeneration of the retina. Our results showed morphological evidence regarding the role of neuronal and glial cells in the pathology of diabetic retinopathy, prior and in addition to microangiopathy. These morphological findings support a neurovascular pathogenesis at the origin of diabetic retinopathy, thus the current treatment approach should be completed by neuroprotective measures.",
keywords = "Journal Article",
author = "J{\'a}nos Feh{\'e}r and Samanta Taurone and Marialuisa Spoletini and Zsolt Bir{\'o} and Bal{\'a}zs Vars{\'a}nyi and Gianluca Scuderi and Orlando, {Maria Patrizia} and Rosaria Turchetta and Alessandra Micera and Marco Artico",
year = "2017",
month = "12",
day = "1",
doi = "10.1177/0394632017748841",
language = "English",
pages = "394632017748841",
journal = "International Journal of Immunopathology and Pharmacology",
issn = "0394-6320",
publisher = "Biomedical Research Press s.a.s.",

}

TY - JOUR

T1 - Ultrastructure of neurovascular changes in human diabetic retinopathy

AU - Fehér, János

AU - Taurone, Samanta

AU - Spoletini, Marialuisa

AU - Biró, Zsolt

AU - Varsányi, Balázs

AU - Scuderi, Gianluca

AU - Orlando, Maria Patrizia

AU - Turchetta, Rosaria

AU - Micera, Alessandra

AU - Artico, Marco

PY - 2017/12/1

Y1 - 2017/12/1

N2 - The previous concept regarding diabetic retinopathy assigned a primary role to hyperglycemia-induced microvascular alterations, while neuronal and glial abnormalities were considered to be secondary to either ischemia or exudation. The aim of this study was to reveal the potential role of neuronal and glial cells in initial and advanced alterations of the retinopathy in human type 2 diabetes. Electron microscopy and histochemical studies were performed on 38 surgically removed human eyes (28 obtained from diabetic patients and 10 from non-diabetic patients). Morphometric analysis of basement membrane material and lipids was performed. An accumulation of metabolic by-products was found in the capillary wall with aging: this aspect was significantly more pronounced in diabetics. Müller glial cells were found to contribute to alterations of the capillary wall and to occlusion, as well as to the development of proliferative retinopathy and cystoid degeneration of the retina. Our results showed morphological evidence regarding the role of neuronal and glial cells in the pathology of diabetic retinopathy, prior and in addition to microangiopathy. These morphological findings support a neurovascular pathogenesis at the origin of diabetic retinopathy, thus the current treatment approach should be completed by neuroprotective measures.

AB - The previous concept regarding diabetic retinopathy assigned a primary role to hyperglycemia-induced microvascular alterations, while neuronal and glial abnormalities were considered to be secondary to either ischemia or exudation. The aim of this study was to reveal the potential role of neuronal and glial cells in initial and advanced alterations of the retinopathy in human type 2 diabetes. Electron microscopy and histochemical studies were performed on 38 surgically removed human eyes (28 obtained from diabetic patients and 10 from non-diabetic patients). Morphometric analysis of basement membrane material and lipids was performed. An accumulation of metabolic by-products was found in the capillary wall with aging: this aspect was significantly more pronounced in diabetics. Müller glial cells were found to contribute to alterations of the capillary wall and to occlusion, as well as to the development of proliferative retinopathy and cystoid degeneration of the retina. Our results showed morphological evidence regarding the role of neuronal and glial cells in the pathology of diabetic retinopathy, prior and in addition to microangiopathy. These morphological findings support a neurovascular pathogenesis at the origin of diabetic retinopathy, thus the current treatment approach should be completed by neuroprotective measures.

KW - Journal Article

U2 - 10.1177/0394632017748841

DO - 10.1177/0394632017748841

M3 - Article

SP - 394632017748841

JO - International Journal of Immunopathology and Pharmacology

JF - International Journal of Immunopathology and Pharmacology

SN - 0394-6320

ER -