Ultraweak Light Emission Is a Common Response of Bacterial Cells to Chemicophysical Stress

Mauro Maccarrone, Alessandro Finazzi AgrO', Nicola Rosato

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Escherichia coli JM101 cells were subjected to pore-forming electric fields, irradiation with ultraviolet light or oxidative stress by either the lipoxygenase products 9- and 13-hydroperoxyoctadecadienoic acids (9- and 13-HPOD) or hydrogen peroxide. It was found that all chemico-physical stresses enhanced ultraweak light emission from the bacterial cells, the most effective treatment being electroporation (up to 20-fold increase in luminescence compared to the control value), followed by oxidative stress with 9- or 13-HPOD (up to 4-fold increase) and irradiation with UV light (up to 2.8-fold increase). Bacterial luminescence was always in the red edge of the spectrum and was paralleled by changes in membrane oxidative index and specific activity of catalase and superoxide dismutase.

Original languageEnglish
Pages (from-to)287-293
Number of pages7
JournalLuminescence
Volume13
Issue number5
Publication statusPublished - Sep 1998

Fingerprint

Oxidative stress
Light emission
Ultraviolet Rays
Luminescence
Oxidative Stress
Irradiation
Light
Electroporation
Lipoxygenase
Ultraviolet radiation
Catalase
Escherichia coli
Hydrogen Peroxide
Superoxide Dismutase
Electric fields
Membranes
Acids
9-hydroperoxy-10,12-octadecadienoic acid
13-hydroperoxy-9,11-octadecadienoic acid

Keywords

  • Catalase
  • Electroporation
  • Oxidative stress
  • Superoxide dismutase
  • UV light

ASJC Scopus subject areas

  • Biophysics
  • Chemistry (miscellaneous)

Cite this

Ultraweak Light Emission Is a Common Response of Bacterial Cells to Chemicophysical Stress. / Maccarrone, Mauro; AgrO', Alessandro Finazzi; Rosato, Nicola.

In: Luminescence, Vol. 13, No. 5, 09.1998, p. 287-293.

Research output: Contribution to journalArticle

Maccarrone, Mauro ; AgrO', Alessandro Finazzi ; Rosato, Nicola. / Ultraweak Light Emission Is a Common Response of Bacterial Cells to Chemicophysical Stress. In: Luminescence. 1998 ; Vol. 13, No. 5. pp. 287-293.
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