UMI, a novel RNF168 ubiquitin binding domain involved in the DNA damage signaling pathway

Sabrina Pinato, Marco Gatti, Cristina Scandiuzzi, Stefano Confalonieri, Lorenza Penengo

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Ubiquitination regulates important cellular processes, including the DNA damage response (DDR) and DNA repair. The complexity of the ubiquitin-mediated signals is decoded by ubiquitin receptors, which contain protein modules named ubiquitin binding domains (UBDs). We previously identified a new ubiquitin ligase, RNF168, involved in DDR and endowed with two UBDs named MIU (motif interacting with ubiquitin). Here we have provided the identification of a novel UBD, the UMI (UIM- and MIU-related UBD), present in RNF168, and characterized the interaction surface with ubiquitin, centered on two Leu residues. We have demonstrated that integrity of the UMI, in addition to the MIUs, is necessary for the proper localization of RNF168 and for ubiquitination of nuclear proteins, including histone H2A. Finally, we have shown that simultaneous inactivation of UMI and MIUs prevents the recruitment to DDR foci of the crucial downstream mediator 53BP1.

Original languageEnglish
Pages (from-to)118-126
Number of pages9
JournalMolecular and Cellular Biology
Volume31
Issue number1
DOIs
Publication statusPublished - Jan 2011

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Ubiquitin
DNA Damage
Ubiquitination
Ligases
Nuclear Proteins
DNA Repair
Histones

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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UMI, a novel RNF168 ubiquitin binding domain involved in the DNA damage signaling pathway. / Pinato, Sabrina; Gatti, Marco; Scandiuzzi, Cristina; Confalonieri, Stefano; Penengo, Lorenza.

In: Molecular and Cellular Biology, Vol. 31, No. 1, 01.2011, p. 118-126.

Research output: Contribution to journalArticle

Pinato, S, Gatti, M, Scandiuzzi, C, Confalonieri, S & Penengo, L 2011, 'UMI, a novel RNF168 ubiquitin binding domain involved in the DNA damage signaling pathway', Molecular and Cellular Biology, vol. 31, no. 1, pp. 118-126. https://doi.org/10.1128/MCB.00818-10
Pinato, Sabrina ; Gatti, Marco ; Scandiuzzi, Cristina ; Confalonieri, Stefano ; Penengo, Lorenza. / UMI, a novel RNF168 ubiquitin binding domain involved in the DNA damage signaling pathway. In: Molecular and Cellular Biology. 2011 ; Vol. 31, No. 1. pp. 118-126.
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