Unconjugated bilirubin modulates the intestinal epithelial barrier function in a human-derived in vitro model

Francesco Raimondi, Valeria Crivaro, Letizia Capasso, Luigi Maiuri, Pasquale Santoro, Maria Tucci, Maria Vittoria Barone, Serena Pappacoda, Roberto Paludetto

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Unconjugated bilirubin promotes intestinal secretion without affecting nutrient digestion or absorption. In the current study, the effects of unconjugated bilirubin (UCB) on the barrier function of the intestinal epithelium were investigated. The apical side of human intestinal cell line Caco-2 monolayers was challenged with purified UCB. Transepithelial electrical resistance and paracellular fluxes of 10 kD Cascade blue conjugate dextran were measured. Cell monolayer viability was studied using LDH release and trypan blue exclusion tests. Redistribution of enterocyte tight junction occludin was studied by confocal microscopy. Bilirubin induced a dose-dependent decrease of transepithelial electrical resistance (TEER). This effect was maximal at 6 h and tended to be reversed at 48 h. Oxidated bilirubin was ineffective. Bilirubin significantly increased fluorescent dextran paracellular passage. Cell viability was not affected by UCB over the 5-200 nmol/L concentration range. Finally, bilirubin triggered a reversible redistribution of tight junctional occludin. UCB increases the permeability of intestinal epithelium. This effect is reversible, dependent on the redox status of the molecule and the rearrangement of the tight junction. These data attribute to bilirubin a novel role of functional modulator of intestinal paracellular permeability in vitro.

Original languageEnglish
Pages (from-to)30-33
Number of pages4
JournalPediatric Research
Volume60
Issue number1
DOIs
Publication statusPublished - Jul 2006

Fingerprint

Bilirubin
Occludin
Tight Junctions
Intestinal Mucosa
Electric Impedance
Permeability
Cell Survival
In Vitro Techniques
Intestinal Secretions
Trypan Blue
Enterocytes
Dextrans
Confocal Microscopy
Oxidation-Reduction
Digestion
Cell Line
Food

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Raimondi, F., Crivaro, V., Capasso, L., Maiuri, L., Santoro, P., Tucci, M., ... Paludetto, R. (2006). Unconjugated bilirubin modulates the intestinal epithelial barrier function in a human-derived in vitro model. Pediatric Research, 60(1), 30-33. https://doi.org/10.1203/01.pdr.0000220344.09034.63

Unconjugated bilirubin modulates the intestinal epithelial barrier function in a human-derived in vitro model. / Raimondi, Francesco; Crivaro, Valeria; Capasso, Letizia; Maiuri, Luigi; Santoro, Pasquale; Tucci, Maria; Barone, Maria Vittoria; Pappacoda, Serena; Paludetto, Roberto.

In: Pediatric Research, Vol. 60, No. 1, 07.2006, p. 30-33.

Research output: Contribution to journalArticle

Raimondi, F, Crivaro, V, Capasso, L, Maiuri, L, Santoro, P, Tucci, M, Barone, MV, Pappacoda, S & Paludetto, R 2006, 'Unconjugated bilirubin modulates the intestinal epithelial barrier function in a human-derived in vitro model', Pediatric Research, vol. 60, no. 1, pp. 30-33. https://doi.org/10.1203/01.pdr.0000220344.09034.63
Raimondi, Francesco ; Crivaro, Valeria ; Capasso, Letizia ; Maiuri, Luigi ; Santoro, Pasquale ; Tucci, Maria ; Barone, Maria Vittoria ; Pappacoda, Serena ; Paludetto, Roberto. / Unconjugated bilirubin modulates the intestinal epithelial barrier function in a human-derived in vitro model. In: Pediatric Research. 2006 ; Vol. 60, No. 1. pp. 30-33.
@article{d60f7fb58b8748648ab0ecd8a77f4fbb,
title = "Unconjugated bilirubin modulates the intestinal epithelial barrier function in a human-derived in vitro model",
abstract = "Unconjugated bilirubin promotes intestinal secretion without affecting nutrient digestion or absorption. In the current study, the effects of unconjugated bilirubin (UCB) on the barrier function of the intestinal epithelium were investigated. The apical side of human intestinal cell line Caco-2 monolayers was challenged with purified UCB. Transepithelial electrical resistance and paracellular fluxes of 10 kD Cascade blue conjugate dextran were measured. Cell monolayer viability was studied using LDH release and trypan blue exclusion tests. Redistribution of enterocyte tight junction occludin was studied by confocal microscopy. Bilirubin induced a dose-dependent decrease of transepithelial electrical resistance (TEER). This effect was maximal at 6 h and tended to be reversed at 48 h. Oxidated bilirubin was ineffective. Bilirubin significantly increased fluorescent dextran paracellular passage. Cell viability was not affected by UCB over the 5-200 nmol/L concentration range. Finally, bilirubin triggered a reversible redistribution of tight junctional occludin. UCB increases the permeability of intestinal epithelium. This effect is reversible, dependent on the redox status of the molecule and the rearrangement of the tight junction. These data attribute to bilirubin a novel role of functional modulator of intestinal paracellular permeability in vitro.",
author = "Francesco Raimondi and Valeria Crivaro and Letizia Capasso and Luigi Maiuri and Pasquale Santoro and Maria Tucci and Barone, {Maria Vittoria} and Serena Pappacoda and Roberto Paludetto",
year = "2006",
month = "7",
doi = "10.1203/01.pdr.0000220344.09034.63",
language = "English",
volume = "60",
pages = "30--33",
journal = "Pediatric Research",
issn = "0031-3998",
publisher = "Lippincott Williams and Wilkins",
number = "1",

}

TY - JOUR

T1 - Unconjugated bilirubin modulates the intestinal epithelial barrier function in a human-derived in vitro model

AU - Raimondi, Francesco

AU - Crivaro, Valeria

AU - Capasso, Letizia

AU - Maiuri, Luigi

AU - Santoro, Pasquale

AU - Tucci, Maria

AU - Barone, Maria Vittoria

AU - Pappacoda, Serena

AU - Paludetto, Roberto

PY - 2006/7

Y1 - 2006/7

N2 - Unconjugated bilirubin promotes intestinal secretion without affecting nutrient digestion or absorption. In the current study, the effects of unconjugated bilirubin (UCB) on the barrier function of the intestinal epithelium were investigated. The apical side of human intestinal cell line Caco-2 monolayers was challenged with purified UCB. Transepithelial electrical resistance and paracellular fluxes of 10 kD Cascade blue conjugate dextran were measured. Cell monolayer viability was studied using LDH release and trypan blue exclusion tests. Redistribution of enterocyte tight junction occludin was studied by confocal microscopy. Bilirubin induced a dose-dependent decrease of transepithelial electrical resistance (TEER). This effect was maximal at 6 h and tended to be reversed at 48 h. Oxidated bilirubin was ineffective. Bilirubin significantly increased fluorescent dextran paracellular passage. Cell viability was not affected by UCB over the 5-200 nmol/L concentration range. Finally, bilirubin triggered a reversible redistribution of tight junctional occludin. UCB increases the permeability of intestinal epithelium. This effect is reversible, dependent on the redox status of the molecule and the rearrangement of the tight junction. These data attribute to bilirubin a novel role of functional modulator of intestinal paracellular permeability in vitro.

AB - Unconjugated bilirubin promotes intestinal secretion without affecting nutrient digestion or absorption. In the current study, the effects of unconjugated bilirubin (UCB) on the barrier function of the intestinal epithelium were investigated. The apical side of human intestinal cell line Caco-2 monolayers was challenged with purified UCB. Transepithelial electrical resistance and paracellular fluxes of 10 kD Cascade blue conjugate dextran were measured. Cell monolayer viability was studied using LDH release and trypan blue exclusion tests. Redistribution of enterocyte tight junction occludin was studied by confocal microscopy. Bilirubin induced a dose-dependent decrease of transepithelial electrical resistance (TEER). This effect was maximal at 6 h and tended to be reversed at 48 h. Oxidated bilirubin was ineffective. Bilirubin significantly increased fluorescent dextran paracellular passage. Cell viability was not affected by UCB over the 5-200 nmol/L concentration range. Finally, bilirubin triggered a reversible redistribution of tight junctional occludin. UCB increases the permeability of intestinal epithelium. This effect is reversible, dependent on the redox status of the molecule and the rearrangement of the tight junction. These data attribute to bilirubin a novel role of functional modulator of intestinal paracellular permeability in vitro.

UR - http://www.scopus.com/inward/record.url?scp=33748085723&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748085723&partnerID=8YFLogxK

U2 - 10.1203/01.pdr.0000220344.09034.63

DO - 10.1203/01.pdr.0000220344.09034.63

M3 - Article

C2 - 16690950

AN - SCOPUS:33748085723

VL - 60

SP - 30

EP - 33

JO - Pediatric Research

JF - Pediatric Research

SN - 0031-3998

IS - 1

ER -