Under the ROS... Thiol network is the principal suspect for autophagy commitment

Giuseppe Filomeni, Enrico Desideri, Simone Cardaci, Giuseppe Rotilio, Maria Rosa Ciriolo

Research output: Contribution to journalArticle

Abstract

Low molecular weight and protein sulphydryls undergo reactive oxygen species (ROS)-mediated oxidation. However, in contrast to the irreversible damages that oxidative conditions yield on biomolecules, the oxidation of reactive cysteines frequently results in reversible modifications, which represent the prototype of the molecular mechanisms underlying redox signaling. Many proteins involved in a wide range of cellular processes have been classified as "redox-sensitive," thereby modulating their function/activity dependent on the redox state of their critical thiols. Growing evidence from the past few years supports the idea that ROS production also correlates with the occurrence of autophagy. Nonetheless, the cysteine protease Atg4 remains the sole example of a protein whose redox regulation has been completely characterized and demonstrated to be necessary for the progression of autophagy. The principal aim of this commentary is to draw attention to the remarkable number of proteins that can fit the double role of: (i) being involved in autophagy, especially in autophagosome formation and (ii) sensing alterations of the cellular redox state by means of reactive cysteine residues. We will also attempt to provide a hypothetical model to explain the possible functional role of thiols in the occurrence of autophagy and outline a network of redox reactions likely concurring to allow the correct initiation and completion of autophagosomes.

Original languageEnglish
Pages (from-to)999-1005
Number of pages7
JournalAutophagy
Volume6
Issue number7
DOIs
Publication statusPublished - Oct 1 2010

Keywords

  • Autophagy
  • Glutathione
  • Reactive oxygen species
  • Redox signaling
  • Sulphydryls

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Under the ROS... Thiol network is the principal suspect for autophagy commitment'. Together they form a unique fingerprint.

  • Cite this