Underevaluation of HIV-1 plasma viral load by a commercially available assay in a cluster of patients infected with HIV-1 A/G circulating recombinant form (CRF02)

Alessandra Amendola, Licia Bordi, Claudio Angeletti, Ubaldo Visco-Comandini, Isabella Abbate, Giuseppina Cappiello, Mohamed A. Budabbus, Osama A. Eljhawi, Mahdi I. Mehabresh, Enrico Girardi, Andrea Antinori, Giuseppe Ippolito, Maria R. Capobianchi

Research output: Contribution to journalArticlepeer-review

Abstract

The authors studied the correlation and agreement of commercially available assays in detection and quantification of the HIV-1 intersubtype A/G circulating recombinant form CRF02. The assays under comparison were Bayer Versant HIV-1 RNA, version 3.0; Roche Amplicor HIV-1 Monitor, version 1.5 (standard procedure); and Organon Teknika NucliSens HIV-1 RNA QT. Plasma samples from 114 patients infected with CRF02 were tested by the three assays under standard conditions. Although correlation among the assays was high and statistically significant for subtype B and CRF02, in the latter instance, NucliSens measured average viral load values (3.29 ± 0.71 log10 copies/mL) about 4 and >8 times lower than those obtained by Versant (3.90 ± 0.90 log10 copies/mL) and Amplicor (4.22 ± 1.05 log10 copies/mL), respectively. Furthermore, in a statistically significant percentage of CRF02-harboring samples, NucliSens produced viral load values undetectable or 1 log10 lower than those obtained in Versant and Amplicor assays. Altogether, these data underline a low performance of NucliSens in detecting and quantifying viremia in plasma samples harboring the CRF02. These results are potentially important as global distribution of new HIV-1 subtypes is expanding, and recombinant strains, particularly CRF02, are emerging and becoming highly prevalent.

Original languageEnglish
Pages (from-to)488-494
Number of pages7
JournalJournal of Acquired Immune Deficiency Syndromes
Volume31
Issue number5
Publication statusPublished - Dec 15 2002

Keywords

  • A/G recombinant HIV-1
  • Bayer Versant
  • Circulating recombinant form (CRF)
  • HIV-1 subtypes
  • Organon Teknika NucliSens
  • Roche Amplicor Monitor
  • Viral load

ASJC Scopus subject areas

  • Immunology
  • Virology

Fingerprint

Dive into the research topics of 'Underevaluation of HIV-1 plasma viral load by a commercially available assay in a cluster of patients infected with HIV-1 A/G circulating recombinant form (CRF02)'. Together they form a unique fingerprint.

Cite this