Understanding biological dynamics: Following cells and molecules to track functions and mechanisms

A. Palamidessi, I. Testa, E. Frittoli, S. Barozzi, M. Garrè, D. Mazza, P. P. Di Fiore, A. Diaspro, G. Scita, Mario Faretta

Research output: Contribution to journalArticlepeer-review

Abstract

The dissection of the molecular circuitries at the base of cell life and the identification of their abnormal transformation during carcinogenesis rely on the characterization of biological phenotypes generated by targeted overexpression or deletion of gene products through genetic manipulation. Fluorescence microscopy provides a wide variety of tools to monitor cell life with minimal perturbations. The observation of living cells requires the selection of a correct balance between temporal, spatial and "statistical" resolution according to the process to be analyzed. In the following paper ad hoc developed optical tools for dynamical tracking from cellular to molecular resolution will be presented. Particular emphasis will be devoted to discuss how to exploit light-matter interaction to selectively target specific molecular species, understanding the relationships between their intracellular compartmentalization and function.

Original languageEnglish
Pages (from-to)947-957
Number of pages11
JournalEuropean Biophysics Journal
Volume39
Issue number6
DOIs
Publication statusPublished - May 2010

Keywords

  • Cell tracking
  • Fluorescence microscopy
  • Fluorescence recovery after photobleaching
  • Photoactivation
  • Two-photon microscopy

ASJC Scopus subject areas

  • Biophysics

Fingerprint Dive into the research topics of 'Understanding biological dynamics: Following cells and molecules to track functions and mechanisms'. Together they form a unique fingerprint.

Cite this