Unfolded HLA class I α chains and their use in an assay of HLA class-I-peptide binding

Nobuyuki Tanigaki, Doriana Fruci, Alberto Chersi, Richard H. Butler

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Unfolded HLA class I α chains were isolated from B-cell lysates by alkaline denaturation and subsequent gel filtration and used for the detection of HLA class-I-peptide binding. Binding to specific peptides in the presence of excess β2-microglobulin induced the unfolded α chains to refold and acquire a conformation that is specific to folded α chains. This conformational change was measured by a specific RIA that involves inhibition of the binding of 125I-labeled HLA-A2 α/β dimers and rabbit anti-HLA-B7 serum absorbed with β2-microglobulin. This assay procedure does not require labeling of either test peptides or test class I proteins and does not seem to have specificity degeneracy. It is applicable to the detection of peptide binding by all HLA class I allelic proteins. Evaluation of the assay conditions and HLA allelic specificity of the peptide binding defined by the use of synthetic peptides are described here, including the technical details, specificity, and reproducibility.

Original languageEnglish
Pages (from-to)119-127
Number of pages9
JournalHuman Immunology
Volume36
Issue number2
DOIs
Publication statusPublished - 1993

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Peptides
HLA-B7 Antigen
HLA-A2 Antigen
Gel Chromatography
Proteins
B-Lymphocytes
Rabbits
Serum

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Unfolded HLA class I α chains and their use in an assay of HLA class-I-peptide binding. / Tanigaki, Nobuyuki; Fruci, Doriana; Chersi, Alberto; Butler, Richard H.

In: Human Immunology, Vol. 36, No. 2, 1993, p. 119-127.

Research output: Contribution to journalArticle

Tanigaki, Nobuyuki ; Fruci, Doriana ; Chersi, Alberto ; Butler, Richard H. / Unfolded HLA class I α chains and their use in an assay of HLA class-I-peptide binding. In: Human Immunology. 1993 ; Vol. 36, No. 2. pp. 119-127.
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