Unilineage hematopoietic differentiation in bulk and single cell culture.

B. Ziegler, U. Testa, G. Condorelli, L. Vitelli, M. Valtieri, C. Peschle

Research output: Contribution to journalArticle

Abstract

The rarity of hematopoietic stem and progenitor cells (HSCs, HPCs) has hampered the analysis of cellular and molecular mechanisms underlying early hematopoiesis. Methodology for HPC purification has partially offset this limitation. A further hurdle has been represented by the heterogeneity of the analyzed HPC/precursor populations: recently, development of unilineage HPC differentiation cultures has provided homogeneous populations of hematopoietic cells, particularly in the early differentiation state, i.e., populations pertaining to a single lineage and a restricted stage of differentiation/maturation, but sufficiently large for cellular/molecular analysis. This report focuses on the development and characterization of the unilineage HPC differentiation culture systems. A section is devoted to selected cellular and molecular mechanisms underlying hematopoiesis, which have been investigated by the HPC unilineage culture approach. Finally, recent advances in the development of HPC unilineage cultures at single cell level are discussed.

Original languageEnglish
Pages (from-to)51-73
Number of pages23
JournalStem Cells
Volume16 Suppl 1
Publication statusPublished - 1998

Fingerprint

Cell Culture Techniques
Hematopoiesis
Hematopoietic Stem Cells
Population

ASJC Scopus subject areas

  • Cell Biology

Cite this

Ziegler, B., Testa, U., Condorelli, G., Vitelli, L., Valtieri, M., & Peschle, C. (1998). Unilineage hematopoietic differentiation in bulk and single cell culture. Stem Cells, 16 Suppl 1, 51-73.

Unilineage hematopoietic differentiation in bulk and single cell culture. / Ziegler, B.; Testa, U.; Condorelli, G.; Vitelli, L.; Valtieri, M.; Peschle, C.

In: Stem Cells, Vol. 16 Suppl 1, 1998, p. 51-73.

Research output: Contribution to journalArticle

Ziegler, B, Testa, U, Condorelli, G, Vitelli, L, Valtieri, M & Peschle, C 1998, 'Unilineage hematopoietic differentiation in bulk and single cell culture.', Stem Cells, vol. 16 Suppl 1, pp. 51-73.
Ziegler B, Testa U, Condorelli G, Vitelli L, Valtieri M, Peschle C. Unilineage hematopoietic differentiation in bulk and single cell culture. Stem Cells. 1998;16 Suppl 1:51-73.
Ziegler, B. ; Testa, U. ; Condorelli, G. ; Vitelli, L. ; Valtieri, M. ; Peschle, C. / Unilineage hematopoietic differentiation in bulk and single cell culture. In: Stem Cells. 1998 ; Vol. 16 Suppl 1. pp. 51-73.
@article{aed8c48ce4f14ef8ba285f8592dd4f31,
title = "Unilineage hematopoietic differentiation in bulk and single cell culture.",
abstract = "The rarity of hematopoietic stem and progenitor cells (HSCs, HPCs) has hampered the analysis of cellular and molecular mechanisms underlying early hematopoiesis. Methodology for HPC purification has partially offset this limitation. A further hurdle has been represented by the heterogeneity of the analyzed HPC/precursor populations: recently, development of unilineage HPC differentiation cultures has provided homogeneous populations of hematopoietic cells, particularly in the early differentiation state, i.e., populations pertaining to a single lineage and a restricted stage of differentiation/maturation, but sufficiently large for cellular/molecular analysis. This report focuses on the development and characterization of the unilineage HPC differentiation culture systems. A section is devoted to selected cellular and molecular mechanisms underlying hematopoiesis, which have been investigated by the HPC unilineage culture approach. Finally, recent advances in the development of HPC unilineage cultures at single cell level are discussed.",
author = "B. Ziegler and U. Testa and G. Condorelli and L. Vitelli and M. Valtieri and C. Peschle",
year = "1998",
language = "English",
volume = "16 Suppl 1",
pages = "51--73",
journal = "Stem Cells",
issn = "1066-5099",
publisher = "AlphaMed Press",

}

TY - JOUR

T1 - Unilineage hematopoietic differentiation in bulk and single cell culture.

AU - Ziegler, B.

AU - Testa, U.

AU - Condorelli, G.

AU - Vitelli, L.

AU - Valtieri, M.

AU - Peschle, C.

PY - 1998

Y1 - 1998

N2 - The rarity of hematopoietic stem and progenitor cells (HSCs, HPCs) has hampered the analysis of cellular and molecular mechanisms underlying early hematopoiesis. Methodology for HPC purification has partially offset this limitation. A further hurdle has been represented by the heterogeneity of the analyzed HPC/precursor populations: recently, development of unilineage HPC differentiation cultures has provided homogeneous populations of hematopoietic cells, particularly in the early differentiation state, i.e., populations pertaining to a single lineage and a restricted stage of differentiation/maturation, but sufficiently large for cellular/molecular analysis. This report focuses on the development and characterization of the unilineage HPC differentiation culture systems. A section is devoted to selected cellular and molecular mechanisms underlying hematopoiesis, which have been investigated by the HPC unilineage culture approach. Finally, recent advances in the development of HPC unilineage cultures at single cell level are discussed.

AB - The rarity of hematopoietic stem and progenitor cells (HSCs, HPCs) has hampered the analysis of cellular and molecular mechanisms underlying early hematopoiesis. Methodology for HPC purification has partially offset this limitation. A further hurdle has been represented by the heterogeneity of the analyzed HPC/precursor populations: recently, development of unilineage HPC differentiation cultures has provided homogeneous populations of hematopoietic cells, particularly in the early differentiation state, i.e., populations pertaining to a single lineage and a restricted stage of differentiation/maturation, but sufficiently large for cellular/molecular analysis. This report focuses on the development and characterization of the unilineage HPC differentiation culture systems. A section is devoted to selected cellular and molecular mechanisms underlying hematopoiesis, which have been investigated by the HPC unilineage culture approach. Finally, recent advances in the development of HPC unilineage cultures at single cell level are discussed.

UR - http://www.scopus.com/inward/record.url?scp=0032227588&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032227588&partnerID=8YFLogxK

M3 - Article

C2 - 11012148

AN - SCOPUS:0032227588

VL - 16 Suppl 1

SP - 51

EP - 73

JO - Stem Cells

JF - Stem Cells

SN - 1066-5099

ER -