Uninfected haematopoietic progenitor (CD34+) cells purified from the bone marrow of AIDS patients are committed to apoptotic cell death in culture

M. C. Re, G. Zauli, D. Gibellini, G. Furlini, E. Ramazzotti, P. Monari, S. Ranieri, S. Capitani, M. La Placa

Research output: Contribution to journalArticle

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Abstract

Objective: To determine the mechanism underlying the poor growth in vitro of haematopoietic progenitor cells isolated from HIV-1-infected patients. Method: Apoptotic death in liquid culture of bone-marrow CD34 + cells obtained from 11 HIV-1-seropositive patients and 18 HIV-1-seronegative donors was quantitatively monitored by a flow cytometry procedure. Results: No significant differences in the percentage of apoptotic cells were noted between the two groups immediately after purification. When CD34 + cells were placed in liquid cultures supplemented with 2 ng/ml interleukin-3, the number of apoptotic cells progressively and significantly (P <0.05) increased in all HIV-1-seropositive patients, while it remained constant in HIV-1-seronegative individuals. Although all HIV-1-seropositive patients showed signs of active viral replication in the bone-marrow micro-environment, progenitor CD34+ cells did not show the presence of active and/or latent HIV-1 infection. Results: Our data demonstrate that CD34+ cells isolated from AIDS patients with active HIV-1 replication in bone-marrow accessory cells are committed to apoptotic death without being directly affected by productive infection.

Original languageEnglish
Pages (from-to)1049-1055
Number of pages7
JournalAIDS (London, England)
Volume7
Issue number8
Publication statusPublished - 1993

Fingerprint

Hematopoietic Stem Cells
HIV-1
Acquired Immunodeficiency Syndrome
Cell Death
Bone Marrow
Bone Marrow Cells
Interleukin-3
HIV Infections
Flow Cytometry
Stem Cells
Cell Count
Tissue Donors
Growth
Infection

Keywords

  • Aids patients
  • Apoptosis
  • Cd34 + cells
  • HIV-1

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Re, M. C., Zauli, G., Gibellini, D., Furlini, G., Ramazzotti, E., Monari, P., ... La Placa, M. (1993). Uninfected haematopoietic progenitor (CD34+) cells purified from the bone marrow of AIDS patients are committed to apoptotic cell death in culture. AIDS (London, England), 7(8), 1049-1055.

Uninfected haematopoietic progenitor (CD34+) cells purified from the bone marrow of AIDS patients are committed to apoptotic cell death in culture. / Re, M. C.; Zauli, G.; Gibellini, D.; Furlini, G.; Ramazzotti, E.; Monari, P.; Ranieri, S.; Capitani, S.; La Placa, M.

In: AIDS (London, England), Vol. 7, No. 8, 1993, p. 1049-1055.

Research output: Contribution to journalArticle

Re, MC, Zauli, G, Gibellini, D, Furlini, G, Ramazzotti, E, Monari, P, Ranieri, S, Capitani, S & La Placa, M 1993, 'Uninfected haematopoietic progenitor (CD34+) cells purified from the bone marrow of AIDS patients are committed to apoptotic cell death in culture', AIDS (London, England), vol. 7, no. 8, pp. 1049-1055.
Re, M. C. ; Zauli, G. ; Gibellini, D. ; Furlini, G. ; Ramazzotti, E. ; Monari, P. ; Ranieri, S. ; Capitani, S. ; La Placa, M. / Uninfected haematopoietic progenitor (CD34+) cells purified from the bone marrow of AIDS patients are committed to apoptotic cell death in culture. In: AIDS (London, England). 1993 ; Vol. 7, No. 8. pp. 1049-1055.
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