Unique epitope of apolipoprotein A-I expressed in pre-β-1 high-density lipoprotein and its role in the catalyzed efflux of cellular cholesterol

P. E. Fielding, M. Kawano, A. L. Catapano, A. Zoppo, S. Marcovina, C. J. Fielding

Research output: Contribution to journalArticlepeer-review

Abstract

The ability of mouse anti-apolipoprotein A-I (apo A-I) monoclonal antibodies to recognize pre-β-HDL species in native plasma was determined. An antibody identifying residues 137-144 of the mature protein uniquely recognized pre-β-1 HDL, an HDL species of low molecular weight implicated in early cholesterol transport from cell membranes to plasma [Castro, G. R., and Fielding, C. J. (1988) Biochemistry 27, 25-29]. Incubation of plasma with this antibody significantly inhibited the efflux of labeled cholesterol from cultured fibroblast monolayers. A second antibody, binding to residues 93-99 of apo A-I, recognized a second pre-β-HDL species (pre-β-2 HDL) but not pre-β-1 HDL and did not inhibit cholesterol efflux. Several other antibodies had broad specificity for HDL (including pre-β-1 HDL). This research suggests that apo A-I residues 137-144 are adjacent to or part of a structural site in pre-β-1 HDL active in promoting the efflux of cellular cholesterol and that this site is not exposed in other HDL species.

Original languageEnglish
Pages (from-to)6981-6985
Number of pages5
JournalBiochemistry
Volume33
Issue number22
DOIs
Publication statusPublished - 1994

ASJC Scopus subject areas

  • Biochemistry

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