Unique epitope of apolipoprotein A-I expressed in pre-β-1 high-density lipoprotein and its role in the catalyzed efflux of cellular cholesterol

P. E. Fielding, M. Kawano, A. L. Catapano, A. Zoppo, S. Marcovina, C. J. Fielding

Research output: Contribution to journalArticle

Abstract

The ability of mouse anti-apolipoprotein A-I (apo A-I) monoclonal antibodies to recognize pre-β-HDL species in native plasma was determined. An antibody identifying residues 137-144 of the mature protein uniquely recognized pre-β-1 HDL, an HDL species of low molecular weight implicated in early cholesterol transport from cell membranes to plasma [Castro, G. R., and Fielding, C. J. (1988) Biochemistry 27, 25-29]. Incubation of plasma with this antibody significantly inhibited the efflux of labeled cholesterol from cultured fibroblast monolayers. A second antibody, binding to residues 93-99 of apo A-I, recognized a second pre-β-HDL species (pre-β-2 HDL) but not pre-β-1 HDL and did not inhibit cholesterol efflux. Several other antibodies had broad specificity for HDL (including pre-β-1 HDL). This research suggests that apo A-I residues 137-144 are adjacent to or part of a structural site in pre-β-1 HDL active in promoting the efflux of cellular cholesterol and that this site is not exposed in other HDL species.

Original languageEnglish
Pages (from-to)6981-6985
Number of pages5
JournalBiochemistry
Volume33
Issue number22
DOIs
Publication statusPublished - 1994

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Apolipoprotein A-I
Epitopes
Cholesterol
Antibodies
Plasmas
HDL2 Lipoprotein
Biochemistry
Fibroblasts
Cell membranes
Monolayers
Molecular Weight
Molecular weight
Monoclonal Antibodies
Cell Membrane
high density lipoprotein-1
Research
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Unique epitope of apolipoprotein A-I expressed in pre-β-1 high-density lipoprotein and its role in the catalyzed efflux of cellular cholesterol. / Fielding, P. E.; Kawano, M.; Catapano, A. L.; Zoppo, A.; Marcovina, S.; Fielding, C. J.

In: Biochemistry, Vol. 33, No. 22, 1994, p. 6981-6985.

Research output: Contribution to journalArticle

Fielding, P. E. ; Kawano, M. ; Catapano, A. L. ; Zoppo, A. ; Marcovina, S. ; Fielding, C. J. / Unique epitope of apolipoprotein A-I expressed in pre-β-1 high-density lipoprotein and its role in the catalyzed efflux of cellular cholesterol. In: Biochemistry. 1994 ; Vol. 33, No. 22. pp. 6981-6985.
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