Abstract
The effect of human recombinant tumor necrosis factor (rhTNF-alpha) on the in vitro colony growth of normal hematopoietic progenitor cells was investigated. In a clonal colony-assay a dose-dependent inhibition of erythroid BFU-E. granulocyte/monocyte CFU-GM and megakaryocyte CFU-Mk was demonstrated. CFU-Mk were completely inhibited by low doses of TNF-alpha (3 U-300 U/ml). 50% inhibition occurred at 10 U/ml of TNF for CFU-Mk, 100% inhibition at 300 U/ml of TNF. 50% inhibition occured at 233 U/ml of TNF for CFU-GM, and 100% inhibition for CFU-GM was not observed. For inhibition of BFU-E higher doses of TNF-alpha were neccessary (100 U-1,000 U/ml). The growth inhibitory effect could selectively be abolished by antibodies against TNF-alpha. Removal of adherent cells and T-lymphocytes from the bone marrow cells had no significant influence of the suppressive effect of TNF-alpha. The inhibitory effect of TNF-alpha is due to a direct action on hematopoietic progenitor cells and not mediated by accessory cells.
Original language | German |
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Pages (from-to) | 109-115 |
Number of pages | 7 |
Journal | Oncology Research and Treatment |
Volume | 12 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1989 |
Keywords
- BFU-E
- CFU
- CFU-Mk
- GM
- Human hematopoietic progenitor cells
- Recombinant human tumor necrosis factor (rhTNF-alpha)
ASJC Scopus subject areas
- Cancer Research
- Hematology
- Oncology