Abstract
B lymphocytes express various different types of surface immunoglobulins that are largely unrelated to other hematological lines, although some reports have described a relationship between malignant B cells and other cells such as macrophages. Multiple genes of hematopoietic lineage, including transcription factors, are co-expressed in hematopoietic stem cells and progenitors, a phenomenon referred to as "lineage priming". Changes in the expression levels and timing of transcription factors can induce the lineage conversion of committed cells, which indicates that the regulation of transcription factors might be particularly critical for maintaining hierarchical hematopoietic development. The aim of this study was to evaluate the surface markers of particular IgM-positive and irregularly nucleated cells detected in patients with inflammatory bowel disease (IBD), and to assess their association with diagnosis and inflammatory cell recruitment. Small intestine, colon and rectal biopsy specimens of 96 IBD patients were studied. Immunoglobulin-producing cells (IPCs) were analyzed by means of immunofluorescence using polyclonal rabbit anti-human Ig and goat anti-human IgM. The specimens positive for B cells with irregular nuclei were assessed using monoclonal antibodies specific for CD79, and λ and κ chains in order to confirm their B cell nature. CD15+ cells, an important marker of inflammatory cell recruitment, were also evaluated. Statistical correlations were sought between the histological findings and clinical expression.34 (35.4%) of the 96 patients (64 with ulcerative colitis and 32 with Crohn's disease) presented a periglandial localization of IPCs with irregular nuclei, which showed surface markers specific for the B cell subset, such as IgM and CD79, but quantitative differences in λ and κ chains. These specimens also contained CD15-positive cells, which are usually absent in healthy controls. The quantitative aspects and localization of the CD15-positive cells correlated with the distribution of the IPCs with irregular nuclei. IPCs with irregular nuclei were significantly more frequent in those patients with Crohn's disease than in those with ulcerative colitis (p
| Original language | English |
|---|---|
| Pages (from-to) | 387-391 |
| Number of pages | 5 |
| Journal | Pathology Research and Practice |
| Volume | 208 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Jul 15 2012 |
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Keywords
- B-1 B cells
- CD15+ cells
- Inflammation
- Inflammatory bowel disease
- Lymphocyte homing
- Lymphocytes
- Mucosal immunity
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Cell Biology
Cite this
Unusual B cell morphology in inflammatory bowel disease. / Defendenti, Caterina; Grosso, Silvia; Atzeni, Fabiola; Croce, Annamaria; Senesi, Olivia; Saibeni, Simone; Bollani, Simona; Almasio, Piero Luigi; Bruno, Savino; Sarzi-Puttini, Piercarlo.
In: Pathology Research and Practice, Vol. 208, No. 7, 15.07.2012, p. 387-391.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Unusual B cell morphology in inflammatory bowel disease
AU - Defendenti, Caterina
AU - Grosso, Silvia
AU - Atzeni, Fabiola
AU - Croce, Annamaria
AU - Senesi, Olivia
AU - Saibeni, Simone
AU - Bollani, Simona
AU - Almasio, Piero Luigi
AU - Bruno, Savino
AU - Sarzi-Puttini, Piercarlo
PY - 2012/7/15
Y1 - 2012/7/15
N2 - B lymphocytes express various different types of surface immunoglobulins that are largely unrelated to other hematological lines, although some reports have described a relationship between malignant B cells and other cells such as macrophages. Multiple genes of hematopoietic lineage, including transcription factors, are co-expressed in hematopoietic stem cells and progenitors, a phenomenon referred to as "lineage priming". Changes in the expression levels and timing of transcription factors can induce the lineage conversion of committed cells, which indicates that the regulation of transcription factors might be particularly critical for maintaining hierarchical hematopoietic development. The aim of this study was to evaluate the surface markers of particular IgM-positive and irregularly nucleated cells detected in patients with inflammatory bowel disease (IBD), and to assess their association with diagnosis and inflammatory cell recruitment. Small intestine, colon and rectal biopsy specimens of 96 IBD patients were studied. Immunoglobulin-producing cells (IPCs) were analyzed by means of immunofluorescence using polyclonal rabbit anti-human Ig and goat anti-human IgM. The specimens positive for B cells with irregular nuclei were assessed using monoclonal antibodies specific for CD79, and λ and κ chains in order to confirm their B cell nature. CD15+ cells, an important marker of inflammatory cell recruitment, were also evaluated. Statistical correlations were sought between the histological findings and clinical expression.34 (35.4%) of the 96 patients (64 with ulcerative colitis and 32 with Crohn's disease) presented a periglandial localization of IPCs with irregular nuclei, which showed surface markers specific for the B cell subset, such as IgM and CD79, but quantitative differences in λ and κ chains. These specimens also contained CD15-positive cells, which are usually absent in healthy controls. The quantitative aspects and localization of the CD15-positive cells correlated with the distribution of the IPCs with irregular nuclei. IPCs with irregular nuclei were significantly more frequent in those patients with Crohn's disease than in those with ulcerative colitis (p
AB - B lymphocytes express various different types of surface immunoglobulins that are largely unrelated to other hematological lines, although some reports have described a relationship between malignant B cells and other cells such as macrophages. Multiple genes of hematopoietic lineage, including transcription factors, are co-expressed in hematopoietic stem cells and progenitors, a phenomenon referred to as "lineage priming". Changes in the expression levels and timing of transcription factors can induce the lineage conversion of committed cells, which indicates that the regulation of transcription factors might be particularly critical for maintaining hierarchical hematopoietic development. The aim of this study was to evaluate the surface markers of particular IgM-positive and irregularly nucleated cells detected in patients with inflammatory bowel disease (IBD), and to assess their association with diagnosis and inflammatory cell recruitment. Small intestine, colon and rectal biopsy specimens of 96 IBD patients were studied. Immunoglobulin-producing cells (IPCs) were analyzed by means of immunofluorescence using polyclonal rabbit anti-human Ig and goat anti-human IgM. The specimens positive for B cells with irregular nuclei were assessed using monoclonal antibodies specific for CD79, and λ and κ chains in order to confirm their B cell nature. CD15+ cells, an important marker of inflammatory cell recruitment, were also evaluated. Statistical correlations were sought between the histological findings and clinical expression.34 (35.4%) of the 96 patients (64 with ulcerative colitis and 32 with Crohn's disease) presented a periglandial localization of IPCs with irregular nuclei, which showed surface markers specific for the B cell subset, such as IgM and CD79, but quantitative differences in λ and κ chains. These specimens also contained CD15-positive cells, which are usually absent in healthy controls. The quantitative aspects and localization of the CD15-positive cells correlated with the distribution of the IPCs with irregular nuclei. IPCs with irregular nuclei were significantly more frequent in those patients with Crohn's disease than in those with ulcerative colitis (p
KW - B-1 B cells
KW - CD15+ cells
KW - Inflammation
KW - Inflammatory bowel disease
KW - Lymphocyte homing
KW - Lymphocytes
KW - Mucosal immunity
UR - http://www.scopus.com/inward/record.url?scp=84863833208&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84863833208&partnerID=8YFLogxK
U2 - 10.1016/j.prp.2012.05.003
DO - 10.1016/j.prp.2012.05.003
M3 - Article
C2 - 22658383
AN - SCOPUS:84863833208
VL - 208
SP - 387
EP - 391
JO - Pathology Research and Practice
JF - Pathology Research and Practice
SN - 0344-0338
IS - 7
ER -