Unusual B cell morphology in inflammatory bowel disease

Caterina Defendenti, Silvia Grosso, Fabiola Atzeni, Annamaria Croce, Olivia Senesi, Simone Saibeni, Simona Bollani, Piero Luigi Almasio, Savino Bruno, Piercarlo Sarzi-Puttini

Research output: Contribution to journalArticlepeer-review

Abstract

B lymphocytes express various different types of surface immunoglobulins that are largely unrelated to other hematological lines, although some reports have described a relationship between malignant B cells and other cells such as macrophages. Multiple genes of hematopoietic lineage, including transcription factors, are co-expressed in hematopoietic stem cells and progenitors, a phenomenon referred to as "lineage priming". Changes in the expression levels and timing of transcription factors can induce the lineage conversion of committed cells, which indicates that the regulation of transcription factors might be particularly critical for maintaining hierarchical hematopoietic development. The aim of this study was to evaluate the surface markers of particular IgM-positive and irregularly nucleated cells detected in patients with inflammatory bowel disease (IBD), and to assess their association with diagnosis and inflammatory cell recruitment. Small intestine, colon and rectal biopsy specimens of 96 IBD patients were studied. Immunoglobulin-producing cells (IPCs) were analyzed by means of immunofluorescence using polyclonal rabbit anti-human Ig and goat anti-human IgM. The specimens positive for B cells with irregular nuclei were assessed using monoclonal antibodies specific for CD79, and λ and κ chains in order to confirm their B cell nature. CD15+ cells, an important marker of inflammatory cell recruitment, were also evaluated. Statistical correlations were sought between the histological findings and clinical expression.34 (35.4%) of the 96 patients (64 with ulcerative colitis and 32 with Crohn's disease) presented a periglandial localization of IPCs with irregular nuclei, which showed surface markers specific for the B cell subset, such as IgM and CD79, but quantitative differences in λ and κ chains. These specimens also contained CD15-positive cells, which are usually absent in healthy controls. The quantitative aspects and localization of the CD15-positive cells correlated with the distribution of the IPCs with irregular nuclei. IPCs with irregular nuclei were significantly more frequent in those patients with Crohn's disease than in those with ulcerative colitis (p

Original languageEnglish
Pages (from-to)387-391
Number of pages5
JournalPathology Research and Practice
Volume208
Issue number7
DOIs
Publication statusPublished - Jul 15 2012

Keywords

  • B-1 B cells
  • CD15+ cells
  • Inflammation
  • Inflammatory bowel disease
  • Lymphocyte homing
  • Lymphocytes
  • Mucosal immunity

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology

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