Abstract
In this study, we describe two renal cell carcinomas (RCC) that occurred at the same time in two brothers, yielding information on the carcinogenic process. We used flow cytometry (FCM) to evaluate nuclear DNA content, and performed cytogenetic analysis. We also carried out fluorescence in situ hybridization (FISH) with a panel of centromeric probes for chromosomes 3, 7, 8, 9, 12, 17, 20, and Y in interphase cells. Flow cytometry analysis revealed diploid histograms in the tumor and 'nonmalignant' samples of patient 1, while an aneuploid cell subpopulation was found in the tumor and 'nonmalignant' samples of patient 2. Tumor samples from the two brothers were studied by FISH, and had common numerical chromosome aberrations: trisomy of chromosomes 3 and 7, and monosomy and trisomy of chromosomes 9 and 17. Moreover, in normal samples from both brothers, we found monosomy 9, and in a normal sample from patient 1, monosomy 17. Cytogenetic analysis revealed trisomy 3 in some cells grown from normal kidney tissue of each brother. The identification of the same chromosome alterations in both brothers appears to provide evidence of an unusual process of carcinogenesis, probably due to a common genetic basis. Copyright (C) 1999 Elsevier Science Inc.
| Original language | English |
|---|---|
| Pages (from-to) | 149-155 |
| Number of pages | 7 |
| Journal | Cancer Genetics and Cytogenetics |
| Volume | 112 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jul 15 1999 |
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ASJC Scopus subject areas
- Cancer Research
- Genetics
- Molecular Biology
Cite this
Unusual chromosome patterns of renal cell carcinomas common to two brothers. / De Vita, Raffaele; Eleuteri, Patrizia; Pomponi, Debora; Calugi, Graziella; Nesci, Lorenzo; Grollino, Maria Giuseppa; Gallucci, Michele.
In: Cancer Genetics and Cytogenetics, Vol. 112, No. 2, 15.07.1999, p. 149-155.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Unusual chromosome patterns of renal cell carcinomas common to two brothers
AU - De Vita, Raffaele
AU - Eleuteri, Patrizia
AU - Pomponi, Debora
AU - Calugi, Graziella
AU - Nesci, Lorenzo
AU - Grollino, Maria Giuseppa
AU - Gallucci, Michele
PY - 1999/7/15
Y1 - 1999/7/15
N2 - In this study, we describe two renal cell carcinomas (RCC) that occurred at the same time in two brothers, yielding information on the carcinogenic process. We used flow cytometry (FCM) to evaluate nuclear DNA content, and performed cytogenetic analysis. We also carried out fluorescence in situ hybridization (FISH) with a panel of centromeric probes for chromosomes 3, 7, 8, 9, 12, 17, 20, and Y in interphase cells. Flow cytometry analysis revealed diploid histograms in the tumor and 'nonmalignant' samples of patient 1, while an aneuploid cell subpopulation was found in the tumor and 'nonmalignant' samples of patient 2. Tumor samples from the two brothers were studied by FISH, and had common numerical chromosome aberrations: trisomy of chromosomes 3 and 7, and monosomy and trisomy of chromosomes 9 and 17. Moreover, in normal samples from both brothers, we found monosomy 9, and in a normal sample from patient 1, monosomy 17. Cytogenetic analysis revealed trisomy 3 in some cells grown from normal kidney tissue of each brother. The identification of the same chromosome alterations in both brothers appears to provide evidence of an unusual process of carcinogenesis, probably due to a common genetic basis. Copyright (C) 1999 Elsevier Science Inc.
AB - In this study, we describe two renal cell carcinomas (RCC) that occurred at the same time in two brothers, yielding information on the carcinogenic process. We used flow cytometry (FCM) to evaluate nuclear DNA content, and performed cytogenetic analysis. We also carried out fluorescence in situ hybridization (FISH) with a panel of centromeric probes for chromosomes 3, 7, 8, 9, 12, 17, 20, and Y in interphase cells. Flow cytometry analysis revealed diploid histograms in the tumor and 'nonmalignant' samples of patient 1, while an aneuploid cell subpopulation was found in the tumor and 'nonmalignant' samples of patient 2. Tumor samples from the two brothers were studied by FISH, and had common numerical chromosome aberrations: trisomy of chromosomes 3 and 7, and monosomy and trisomy of chromosomes 9 and 17. Moreover, in normal samples from both brothers, we found monosomy 9, and in a normal sample from patient 1, monosomy 17. Cytogenetic analysis revealed trisomy 3 in some cells grown from normal kidney tissue of each brother. The identification of the same chromosome alterations in both brothers appears to provide evidence of an unusual process of carcinogenesis, probably due to a common genetic basis. Copyright (C) 1999 Elsevier Science Inc.
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UR - http://www.scopus.com/inward/citedby.url?scp=0032994751&partnerID=8YFLogxK
U2 - 10.1016/S0165-4608(98)00262-3
DO - 10.1016/S0165-4608(98)00262-3
M3 - Article
C2 - 10686943
AN - SCOPUS:0032994751
VL - 112
SP - 149
EP - 155
JO - Cancer Genetics and Cytogenetics
JF - Cancer Genetics and Cytogenetics
SN - 0165-4608
IS - 2
ER -