Unusual chromosome patterns of renal cell carcinomas common to two brothers

Raffaele De Vita, Patrizia Eleuteri, Debora Pomponi, Graziella Calugi, Lorenzo Nesci, Maria Giuseppa Grollino, Michele Gallucci

Research output: Contribution to journalArticle

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Abstract

In this study, we describe two renal cell carcinomas (RCC) that occurred at the same time in two brothers, yielding information on the carcinogenic process. We used flow cytometry (FCM) to evaluate nuclear DNA content, and performed cytogenetic analysis. We also carried out fluorescence in situ hybridization (FISH) with a panel of centromeric probes for chromosomes 3, 7, 8, 9, 12, 17, 20, and Y in interphase cells. Flow cytometry analysis revealed diploid histograms in the tumor and 'nonmalignant' samples of patient 1, while an aneuploid cell subpopulation was found in the tumor and 'nonmalignant' samples of patient 2. Tumor samples from the two brothers were studied by FISH, and had common numerical chromosome aberrations: trisomy of chromosomes 3 and 7, and monosomy and trisomy of chromosomes 9 and 17. Moreover, in normal samples from both brothers, we found monosomy 9, and in a normal sample from patient 1, monosomy 17. Cytogenetic analysis revealed trisomy 3 in some cells grown from normal kidney tissue of each brother. The identification of the same chromosome alterations in both brothers appears to provide evidence of an unusual process of carcinogenesis, probably due to a common genetic basis. Copyright (C) 1999 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)149-155
Number of pages7
JournalCancer Genetics and Cytogenetics
Volume112
Issue number2
DOIs
Publication statusPublished - Jul 15 1999

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Renal Cell Carcinoma
Siblings
Monosomy
Chromosomes
Chromosomes, Human, Pair 3
Chromosomes, Human, Pair 7
Cytogenetic Analysis
Trisomy
Fluorescence In Situ Hybridization
Flow Cytometry
Neoplasms
Chromosomes, Human, Pair 17
Interphase
Aneuploidy
Diploidy
Chromosome Aberrations
Carcinogenesis
Kidney
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Unusual chromosome patterns of renal cell carcinomas common to two brothers. / De Vita, Raffaele; Eleuteri, Patrizia; Pomponi, Debora; Calugi, Graziella; Nesci, Lorenzo; Grollino, Maria Giuseppa; Gallucci, Michele.

In: Cancer Genetics and Cytogenetics, Vol. 112, No. 2, 15.07.1999, p. 149-155.

Research output: Contribution to journalArticle

De Vita, R, Eleuteri, P, Pomponi, D, Calugi, G, Nesci, L, Grollino, MG & Gallucci, M 1999, 'Unusual chromosome patterns of renal cell carcinomas common to two brothers', Cancer Genetics and Cytogenetics, vol. 112, no. 2, pp. 149-155. https://doi.org/10.1016/S0165-4608(98)00262-3
De Vita R, Eleuteri P, Pomponi D, Calugi G, Nesci L, Grollino MG et al. Unusual chromosome patterns of renal cell carcinomas common to two brothers. Cancer Genetics and Cytogenetics. 1999 Jul 15;112(2):149-155. https://doi.org/10.1016/S0165-4608(98)00262-3
De Vita, Raffaele ; Eleuteri, Patrizia ; Pomponi, Debora ; Calugi, Graziella ; Nesci, Lorenzo ; Grollino, Maria Giuseppa ; Gallucci, Michele. / Unusual chromosome patterns of renal cell carcinomas common to two brothers. In: Cancer Genetics and Cytogenetics. 1999 ; Vol. 112, No. 2. pp. 149-155.
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